ASTM_D_2330_-_02.pdf

Designation:D 2330 02An American National StandardStandard Test Method forMethylene Blue Active Substances1This standard is issued under the fixed designation D 2330;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon(e)indicates an editorial change since the last revision or reapproval.This standard has been approved for use by agencies of the Department of Defense.1.Scope1.1 This test method covers the determination of com-pounds that react with methylene blue under the conditionsspecified in the test procedure.They are referred to asmethylene blue active substances(MBAS),and are calculatedand reported in terms of the reference material,linear alkylbenzene sulfonate,LAS.1.2 This test method is applicable for determining MBAS inwater and wastewater.It is the users responsibility to ensurethe validity of this test method for waters of untested matrices.1.3 This test method is a simple,rapid,control proceduresuitable for monitoring the effectiveness of a biodegradation orother linear alkyl benzene sulfonate(LAS)removal process.For greater specificity and interference removal,the pretreat-ment procedure in Annex A1 should be used.Data derivedwithout the pretreatment procedure should be interpreted withcare.This test method is applicable in the range from 0.03 to1.5 mg/L for a 100-mL sample.1.4 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.For a specifichazard statement,see 8.3.2.Referenced Documents2.1 ASTM Standards:D 459 Terminology Relating to Soap and Other Detergents2D 1129 Terminology Relating to Water3D 1193 Specification for Reagent Water3D 3370 Practices for Sampling Water from Closed Con-duits3D 3856 Guide for Good Laboratory Practices in Laborato-ries Engaged in Sampling and Analysis of Water3D 4210 Practice for Intralaboratory Quality Control Proce-dures and a Discussion on Reporting Low-Level Data3D 5788 Guide for Spiking Organics into Aqueous Samples4D 5789 Practice for Writing Quality Control Specificationsfor Standard Test Methods for Organic Constituents4D 5847 Practice for Writing Quality Control Specificationsfor Standard Test Methods for Water Analysis4E 60 Practice for Molecular Absorption SpectrometricMethods for Chemical Analysis of Metals,Ores,andRelated Materials5E 131 Terminology Relating to Molecular Spectroscopy6E 275 Practice for Describing and Measuring Performanceof Ultraviolet,Visible,and Near-Infrared Spectrophotom-eters63.Terminology3.1 DefinitionsFor definitions of terms used in this testmethod,refer to Terminology D 1129 and E 131.3.2 Definitions of Terms Specific to This Standard:3.2.1 alkyl benzene sulfonate(ABS)7the generic nameapplied to the neutralized product resulting from the sulfona-tion of a branched-chain alkylated benzene.See also Termi-nology D 459.3.2.2 linear alkyl benzene sulfonate(LAS)7a form of alkylbenzene sulfonate(ABS)in which the alkyl group is linearrather than a branched chain.See also Terminology D 459.4.Summary of Test Method84.1 This test method is based upon the formation of ablue-colored chloroform extractable ion pair by the reaction ofcationic methylene blue and an anionic surfactant(includingLAS,other sulfonates,and sulfate esters).4.2 The sample is mixed with an acidified,aqueous solutionof methylene blue.Any resulting hydrophobic ion pair whichmay be formed is extracted successfully with chloroform.Thecombined chloroform extracts are washed with an acid solutionto remove the less hydrophobic ion pairs(having low partition1This test method is under the jurisdiction of ASTM Committee D19 on Waterand is the direct responsibility of Subcommittee D19.06 on Methods forAnalysis forOrganic Substances in Water.Current edition approved July 10,2002.Published August 2002.Originallypublished as D2330 65 T.Last previous edition D 2330 88(1995)e1.2Annual Book of ASTM Standards,Vol 15.04.3Annual Book of ASTM Standards,Vol 11.01.4Annual Book of ASTM Standards,Vol 11.02.5Annual Book of ASTM Standards,Vol 03.05.6Annual Book of ASTM Standards,Vol 03.06.7For a more complete discussion of terms relating to synthetic detergents andtheir significance,refer to“Syndets and Waste Disposal”by McKinney,R.E.,Sewage and Industrial Wastes,Vol 29,Part 6,June 1957,pp.654666.8Adopted from“Surfactants(Anionic)Methylene Blue Methods,”StandardMethods for the Examination of Water and Waste Water,Twelfth Ed.,1965.1Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959,United States.coefficients)that can be formed by potentially interferingsubstances.The chloroform layer retains the highly hydropho-bic methylene blue-LAS ion pairs.4.3 The inten