ASTM_D_1413_-_07e1.pdf

Designation:D1413071Standard Test Method forWood Preservatives by Laboratory Soil-Block Cultures1This standard is issued under the fixed designation D1413;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1NOTEEditorial corrections were made throughout in April 2008.1.Scope1.1 This test method covers determination of the minimumamount of preservative that prevents decay of selected speciesof wood by selected fungi under optimum laboratory condi-tions.1.2 The requirements for preparation of the material fortesting and the test procedure appear in the following order:Section TitleSectionSummary of Test Method3Significance and Use4Apparatus5Reagents6Wood and Test Blocks7Test Fungi8Culture Media9Preparation of Test Cultures10Preparation and Impregnation of Test Blocks11Conditioning Treated Blocks12Preservative Permanence13Stabilization of Treated Test Blocks and Placement in CultureBottles14Incubation and Duration of Test15Handling Test Blocks After Exposure to Test Fungi16Calculation of Weight Losses17Evaluation of Test Results18Refining the Threshold19Report20Precision and Bias21Keywords221.3 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D841 Specification for Nitration Grade TolueneD1193 Specification for Reagent WaterD1758 Test Method for Evaluating Wood Preservatives byField Tests with Stakes(Withdrawn 2015)3D3345 Test Method for Laboratory Evaluation of Wood andOther Cellulosic Materials for Resistance to TermitesD3507 Test Methods for Penetration of Preservatives inWood and for Differentiating Between Heartwood andSapwood(Withdrawn 2008)3E11 Specification for Woven Wire Test Sieve Cloth and TestSieves2.2 Other Standards:4AWPA E10 Testing Wood Preservatives by Laboratory Soil-Block Cultures3.Summary of Test Method3.1 Conditioned blocks of wood are impregnated withdifferent concentration solutions of a preservative in water orsuitable organic solvent to produce a series of retentions of thepreservatives in the blocks.After periods of conditioning orweathering,the impregnated blocks are exposed to one or morestrains of wood-destroying fungi,one fungus for each testseries.The minimum amount of preservative that in theprescribed testing protects the impregnated blocks againstdecay by a given test fungus is defined as the thresholdretention for that organism.Failure to protect is evidenced byloss of wood from the treated wood blocks,as indicated by aloss in weight.3.2 Provision must be made for coordinated preparation ofthe test cultures and for impregnation,conditioning,or weath-ering and conditioning,of the test blocks.4.Significance and Use4.1 This test method is useful in the development of newwood preservatives and preservative systems by evaluating theminimum preservative retention to prevent decay under labo-ratory conditions.The results are used to facilitate targetretentions in subsequent tests for effectiveness against termites1This test method is under the jurisdiction of ASTM Committee D07 on Woodand is the direct responsibility of Subcommittee D07.06 on Treatments for WoodProducts.Current edition approved April 1,2007.Published April 2007.Originallyapproved in 1949.Last previous edition approved in 2005 as D1413 05b.DOI:10.1520/D1413-07E01.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3The last approved version of this historical standard is referenced onwww.astm.org.4Available from American Wood Protection Association(AWPA),P.O.Box361784,Birmingham,AL 35236-1784,http:/.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1(see Test Method D3345)and in field stakes(see Test MethodD1758).The sections on Treatment and Preservative Perma-nence are referenced by other ASTM standards.The testmethod assumes that the test blocks exposed to certain condi-tions after treatment will achieve equilibrium,and will returnto the same equilibrium after exposure to fungi.This assump-tion may lead to weight loss that is not due to decay.The testuses live cultures of fungal organisms that require carefulcolonization,storage,and feeding to remain viable strains.5.Apparatus5.1 Conditioning Chamber or Room,maintained at aselected temperature between 20 and 30C(68 and 86F)anda selected relative humidity between 25 and 75%.The selectedtemperature shall not vary more than 61C(