Designation:D2800–92(Reapproved1998)StandardTestMethodforPreparationofMethylEstersFromOilsforDeterminationofFattyAcidCompositionbyGas-LiquidChromatography1ThisstandardisissuedunderthefixeddesignationD2800;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(e)indicatesaneditorialchangesincethelastrevisionorreapproval.ThisstandardhasbeenapprovedforusebyagenciesoftheDepartmentofDefense.1.Scope1.1Thistestmethodcoversarapidprocedureforconver-sionofanimalandvegetablefattyoilsintomethylestersofthefattyacidssuitableforanalysisbygas-liquidchromatography.1.2Thistestmethodisbelievedtobeapplicabletomostdryingoilsusedinthepaintindustryincludinglinseed,soya,safflower,andcottonseedoils.Unsaturatedoilswithatendencytoundergoalkalineisomerizationortopolymerizeinthepresenceofborontrifluoride(BF3)maygiveerroneousresults.Unsaponifiablesarenotremoved.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.Forspecifichazardstatements,see5.4andNote1.2.ReferencedDocuments2.1ASTMStandards:D1193SpecificationforReagentWater2D1983TestMethodforFattyAcidCompositionbyGas-LiquidChromatographyofMethylEsters3D2245TestMethodforIdentificationofOilsandOilAcidsinSolvent-ReduciblePaints4D3457TestMethodforPreparationofMethylEstersfromFattyAcidsforDeterminationofFattyAcidCompositionbyGas-LiquidChromatography33.SummaryofTestMethod3.1ThistestmethodisbaseduponarapidsaponificationoftheoilwithmethanolicsodiumhydroxidefollowedbyboilingthesoapswithBF3-methanolinthesamevesseltoconvertquantitativelythefattyacidstomethylesters.Themethylestersarefloatedoutofthemixtureuponadditionofasaturatedsaltsolution.3.2Methylmargaratemaybeaddedquantitativelytotheoilpriortosaponificationandmethylationtose...
