ASTM_D_3345_-_22.pdf

Designation:D334522Standard Test Method forLaboratory Evaluation of Wood for Resistance toSubterranean Termites1This standard is issued under the fixed designation D3345;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method covers the laboratory evaluation oftreated or untreated wood for its resistance to subterraneantermites.This test is considered as a screening test for treatedmaterial and further evaluation by field methods is required.1.2 The values stated in SI units are to be regarded as thestandard.The values given in parentheses are for informationonly.1.3 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.(WarningSee 7.1.4.)1.4 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:2D1413 Test Method for Wood Preservatives by LaboratorySoil-Block Cultures(Withdrawn 2016)32.2 Other Documents:4AWPA E10 Laboratory Method for Evaluating the DecayResistance of Wood-Based MaterialsAgainst Pure Basidi-omycete Cultures:Soil/Block TestAWPA E1 Laboratory Method for Evaluating the TermiteResistance of Wood-Based Materials:Choice and NoChoice Tests3.Choice or No-Choice Methodology3.1 There are two types of basic termite bioassays:3.1.1 Choice testtermites are presented with two feedingtargets which may or may not contain insecticidal components.Difference in mass loss may be indicative of preference oravoidance of one material over another.3.1.2 No-choice testtermites are presented with a singularchoice and mass loss indicates ability of the insect to consumethe material.In no-choice tests,mortality data is needed todetermine dose mortality based on consumption of the testmaterial,which is often treated with suspected insecticidalcompounds.NOTE1It is recommended that both choice and no-choice tests beperformed when evaluating materials for resistance to subterraneantermites as the different tests address different behavioral responses of thetermites.3.2 Details regarding test methodology shall be included inthe final report,and use of choice or no-choice,or both,shallbe indicated in the report title.4.Apparatus4.1 Containers,Clear,Clean Glass or Plastic,with looselyfitting tops with liners removed,220 cm3to 450 cm3(225 mL(8 oz)to 480 mL(16 oz).4.2 Tray,enamel,stainless steel,or plastic,0.25 m by0.51 m(10 in.by 20 in.)and bucket.4.3 Paper Towels or Corrugated Cardboard.4.4 Incubation Room(or Cabinet),maintained at a tempera-ture between 25 C to 28 C(77 F to 82 F).5.Reagents and Materials5.1 Benzalkonium Chloride Solution(1:750)Add 1 partbenzalkonium chloride to 750 parts water.A comparablesurface antiseptic is satisfactory.5.2 Distilled or Deionized Water,heat-sterilized.5.3 Sand,brown or white,screened,washed,and heat-sterilized.1This test method is under the jurisdiction of ASTM Committee D07 on Woodand is the direct responsibility of Subcommittee D07.06 on Treatments for WoodProducts.Current edition approved Feb.1,2022.Published March 2022.Originallyapproved in 1974.Last previous edition approved in 2017 as D3345 17.DOI:10.1520/D3345-22.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3The last approved version of this historical standard is referenced onwww.astm.org.4Available from the American Wood Protection Association(AWPA),PO Box361784,Birmingham,AL 35236-1784,http:/.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.15.4 Southern Yellow Pine(SYP)(Pinus spp.),measuring25.4 mm(1.00 in.)square by 6.4 mm(0.25 in.)in the longitu-dinal or tangential direction.Sapwood,no visible defects,smoothed surfaces equivalent to planed or sanded,2 to 3rings/cm(4 to 6 rings/in.).All test samples shall come fromsame parent board(1,2).55.4.1 Other wood species may be used,but in ea