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TM_D_4211_
_21
Designation:D421121Standard Guide forFish Sampling1This standard is issued under the fixed designation D4211;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope*1.1 This guide covers the use of lethal and non-lethalcollection practices for fish.1.1.1 Lethal practices include the use of rotenone andantimycin which are used to collect or eradicate fish;numerouschemicals have been used but presently only rotenone andantimycin are U.S.Environmental Protection Agency(EPA)-approved for this use.1.2 Non-lethal collection practices typically do not causemortality to fish.1.2.1 Non-lethalpracticesincludesurfaceorbankobservation,underwater observation,gill netting,beach seines,hoop nets,fyke nets,trap nets,electroshocking,minnow traps,enclosure(pop drop and throw)traps,angler surveys,commer-cial surveys.1.3 The focus of this guide is to provide sampling practicesfor fish collection.This standard does not cover the identifica-tion of species or any statistical methods for the sampling data.1.4 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.5 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.Refer to the MSDSs for all chemicals used in this procedure.1.6 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:2D1129 Terminology Relating to WaterD4131 Practice for Sampling Fish with Rotenone3.Terminology3.1 Definitions:3.1.1 For definitions of terms used in this standard,refer toTerminology D1129.3.2 Definitions of Terms Specific to This Standard:3.2.1 rotenone,nan odorless,colorless,crystalline isofla-vone used as a broad-spectrum insecticide,piscicide,andpesticide.3.2.1.1 DiscussionThis chemical is commonly used asnonselective piscicide(fish killer).4.Significance and Use4.1 Fish sampling includes a number of lethal and non-lethal practices.4.2 This guide provides an overview of commonly used fishsampling practices.4.3 This summary serves as a brief accounting of optionsavailable to personnel responsible for determining the fishsampling practice or practices that best serve the samplingobjectives.5.Basis of Classification of Practices5.1 Lethal PracticesThe significance of using chemicalfish toxicants is that more complete population analyses or totaleradication,or both,can be accomplished.Target species canbe selectively eradicated by varying concentrations.Thisprovides a very effective tool in fisheries investigations andmanagement programs.Water conditions(that is,pH,temperature,alkalinity,and so forth)and morphology can belimiting factors.1This guide is under the jurisdiction of ASTM Committee D19 on Water and isthe direct responsibility of Subcommittee D19.24 on Water Microbiology.Current edition approved Sept.1,2021.Published September 2021.Originallyapproved in 1982.Last previous edition approved in 2012 as D4211 82(2012)which was withdrawn January 2021 and reinstated in September 2021.DOI:10.1520/D4211-21.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.*A Summary of Changes section appears at the end of this standardCopyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.15.1.1 RotenoneRotenone used as a fish toxicant is highlyversatile and can be used effectively to collect fish samples;toeradicate fish;and to selectively remove certain fish species(see Practice D4131).5.1.1.1 Its effectiveness is reduced in cold 20C,anddosage required increases with alkalinities.It may also elimi-nate food web organisms.Fish may be repulsed from treatedareas.5.1.2 AntimycinAntimycin is versatile in the selectiveremoval of scalefish or even more selectively against certaincentrarchids(sunfish)and minnows.5.1.2.1 Its effectiveness is re