Designation:D4200−82(Reapproved2011)StandardTestMethodforEvaluatingInhibitoryEffectsofInkGridsonMembraneFilters1ThisstandardisissuedunderthefixeddesignationD4200;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethoddescribesaprocedurewherebytheuserofink-griddedmembranefiltersinwaterqualitystudiescanascertainwhetherornotthegridlinesaretoxicandinhibitorytobacterialgrowthwhenthemembraneanditsentrappedbacteriaareincubatedonasuitablemedia.1.2ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:2D1129TerminologyRelatingtoWaterD1193SpecificationforReagentWater3.Terminology3.1Definitions—Fordefinitionsoftermsusedinthistestmethod,refertoTerminologyD1129.4.SummaryofTestMethod4.1Aheavybacterialsuspensionisfilteredthroughagriddedmembranefilter.Thebacterialconcentrationemployedissufficienttocovermuchofthemembranewithbacterialcolonies.4.2Afterfiltrationthemembraneisincubatedonasuitablemediumandthedistributionofthecoloniesandshapeofthecoloniesnotedintheareaaroundeachgridline.5.SignificanceandUse5.1Thistestmethodmaybeappliedtodeterminethesuitabilityofgrid-markedmembranefiltersforuseinbacte-riologicalculturetechniquesforthedetectionandenumerationofbacterialorganisms.5.2Aparticularlysensitiveorganismandgrowthconditionshavebeenselectedforthistestmethodinordertomaximizesensitivitytotoxicmaterialspossiblypresentintheinksusedforgrid-markingmembranefilters.6.Apparatus6.1Incubator,capableofmaintainingtemperaturesof44.560.2°C.6.2MembraneFi...
