ASTM_E_2753_-_10.pdf

Designation:E275310Standard Specification forEretmocerus eremicus Rose and Zolnerowich(Hymenoptera:Aphelinidae)1This standard is issued under the fixed designation E2753;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This specification describes a method for determiningwhether the quantity and quality of adult Eretmocerus eremicusin a shipment adhere to quantity and quality specifications.Thetest also allows the purity of shipments to be determined.Included are referenced documents,a description of standardterminology,specifications,and the test method.1.2 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.2.Referenced Documents2.1 ASTM Standards:2E2200 Specification for Information Included with Packag-ing of Multi-Cellular Biological Control Organisms(Withdrawn 2010)33.Terminology3.1 name of productEretmocerus eremicus Gahan.3.2 preferred hosts and preyGreenhouse Whitefly,Tri-aleurodes vaporariorum(Westwood),Silverleaf Whitefly,Be-misia argentifolii Bellows and Perring and Tobacco Whitefly,Bemisia tabaci(Gennadius).3.3 life stage shippedpupa within the host insect,T.vaporariorum.3.4 carda sample unit.Pupae of Eretmocerus eremicus aremounted on cardstock strips that are subdivided into“cards”.3.5 package claimthe number of adults expected toemerge from and leave each card.3.6 test statisticthe average number of wasps caughtduring the flight test expressed as a percentage of the packageclaim.3.7 critical valuea number that the test statistic is com-pared to in order to determine whether the quantity and qualityrequirement has been met.Critical values depend on theprobability of error that can be tolerated and on the number ofsamples used in the test.4.Classification4.1 PhylumArthropoda.4.2 ClassInsecta.4.3 OrderHymenoptera.4.4 FamilyAphelinidae.4.5 GenusEretmocerus.4.6 Specieseremicus.5.Summary of Test Method(Determining the Number ofE.eremicus Released from a Shipment and itsConformity to the Standard)5.1 The test describes methods for determining whether thenumber of adult E.eremicus that are capable of flight meets orexceeds the package claim.Amethod of assessing the purity ofa shipment is included.5.2 The quantity of E.eremicus per card and their flightability is determined by counting the number of wasps caughton a sticky trap suspended in a test chamber.Three or more testchambers are used per shipment,each chamber contains onerandomly chosen card with E.eremicus pupae.The numbers ofwasps caught on each sticky card are counted and results areused to calculate the average number of wasps per card.Thisaverage is used in combination with the known samplingdistribution and variation in counts to judge whether specifi-cations are met.Live contaminants are identified and recorded.5.3 Quantity and Quality SpecificationThe test describedin this document is used for both the quantity and qualityassessment.To meet the quantity and quality specificationdefined by the“package claim”,100%of the number of adultsshould be capable of flight as measured by the flight testdescribed herein.1This specification is under the jurisdiction of ASTM Committee E35 onPesticides,Antimicrobials,and Alternative Control Agents and is the directresponsibility of Subcommittee E35.30 on Natural Multi-Cellular(Metazoan)Biological Control Organisms.Current edition approved April 1,2010.Published May 2010.DOI:10.1520/E275310.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3The last approved version of this historical standard is referenced onwww.astm.org.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 5.4 PurityThe purity specification is that shipments,andhence samples,do not contain species other than Eretmoceruseremicus.6.Significance and Use6.1 This method was developed to determine that thenumbers of E.eremicus supplied in a shipment meet thepackage claim and that wasps at receipt have good flightcapability.The application of this method will ensure astandardized evaluation of the product and judicious decisionsabout product compliance to the package claim.7.Materials7.1 Test chamber(6.5 8 cm acrylic tube).7.2 Support wire.7.3 Double-sided dry-stick yellow sticky trap(2.5 4 cm).7.4 Nylon screen(100 micron mesh).7.5 Clear plastic cling-film(see Fig.1).7.6 Headband magnifier(7 to 10)is optional.8.Test Unit8.1 A single shipment of E.eremicus is considered a testunit.9.Pre-Test Conditions9.1 If required,a shipment can be stored for a maximum 24h at 10 to 15C,relative humidity(