Designation:E2783−11StandardTestMethodforAssessmentofAntimicrobialActivityforWaterMiscibleCompoundsUsingaTime-KillProcedure1ThisstandardisissuedunderthefixeddesignationE2783;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethodmeasuresthechangesofapopulationofaerobicandanaerobicmicroorganismswithinaspecificsamplingtimewhentestedagainstantimicrobialtestmaterialsinvitro.Theorganismsusedarestandardizedastogrowthrequirementsandinoculumpreparationandmustgrowundertheconditionsofthetest.Theprimarypurposeofthistestmethodistoprovideasetofstandardizedconditionsandtestorganismstofacilitatecomparativeassessmentsofantimicro-bialmaterialsmiscibleinaqueoussystems.1.2Thistestmethodallowstheoptionofusingatestsamplesizeof10mLor100mL.1.3Knowledgeofmicrobiologicaltechniquesisrequiredforthisprocedure.1.4Aseptictechniqueshouldbepracticedatalltimes.1.5Inthistestmethod,SIunitsareusedforallapplications,exceptfordistanceinwhichcaseinchesareusedandSIunitsfollowinparentheses.1.6Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:2E691PracticeforConductinganInterlaboratoryStudytoDeterminethePrecisionofaTestMethodE1054TestMethodsforEvaluationofInactivatorsofAnti-microbialAgents3.Terminology3.1Definitions:3.1.1antimicrobial,n—describesanagentthatkillsorinactivatesmicroorganismsorsuppressestheirgrowthorreproduction.3.1.2drug,n—articlesintendedforuseinthediagnosis,cure,mitigation,treatment,orpreventionofdiseaseinmanorotheranimals.Drugsareintendedtoaffectthestructureoranyfunctionofthebodyofmanorotheranimals.3.1.3initialmicrobialpopulation,n—bacteri...
