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TM_D_3345_
_08
Designation:D334508Standard Test Method forLaboratory Evaluation of Wood and Other CellulosicMaterials for Resistance to Termites1This standard is issued under the fixed designation D3345;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method covers the laboratory evaluation oftreated or untreated cellulosic material for its resistance tosubterranean termites.This test should be considered as ascreening test for treated material and further evaluation byfield methods is required.1.2 The values stated in SI units are to be regarded as thestandard.The values given in parentheses are for informationonly.1.3 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.(WarningSee6.1.4.)2.Referenced Documents2.1 ASTM Standards:2D1413 Test Method for Wood Preservatives by LaboratorySoil-Block Cultures2.2 Other Documents:3AWPA E10 Testing Wood Preservatives by Laboratory Soil-Block Cultures3.Apparatus3.1 Containers,Glass or Clean Plastic,with loosely fittingtops with liners removed,220 to 450 cm3(8 to 16 oz).3.1.1 If volatile chemicals are to be tested,a 4.8 mm(No.12or approximately316in.)hole is drilled in the center of the top.3.2 Tray,enamel,stainless steel,or plastic,0.25 m by 0.51m(10 by 20 in.)and bucket.3.3 Paper Towels.4.Reagents and Materials4.1 Benzalkonium Chloride Solution(1+750)Add 1 partbenzalkonium chloride to 750 parts water.A comparablesurface antiseptic is satisfactory.4.2 Distilled Water.4.3 Sand,brown or white,screened,washed,and heat-sterilized.4.4 Southern Yellow Pine(SYP)(Pinus spp.)measuring 25.4mm(1.00 in.)square by 6.4 mm(0.25 in.)in the tangentialdirection.Sapwood,no visible defects,smoothed surfacesequivalent to planed or sanded,2 to 3 rings/cm(4 to 6rings/in.).All test samples should come from same parentboard.4.4.1 Other wood species may be used,but in each separatetest using other species as the major test wood,five SYPsapwood blocks should be used as additional controls to permitthe correlation of test results among laboratories.4.5 Subterranean TermitesUse a major common speciesof the region being studied.4.5.1 Specific identification of any termites used shall beobtained and reported with the test data.5.Determination of Sand Water-Holding Capacity5.1 Determine the quantity of distilled water to be added tothe sand during the test as follows:5.1.1 Place 100 g of oven-dry sand in a beaker anddetermine the volume of water required to saturate the sand.The saturation point is defined as the point when the additionof more water will result in free water on the surface of thesand.5.1.2 Calculate the percent saturation as follows:%Saturation5weight of water/oven dry weight of sand!3100(1)5.1.3 Add water to the sand as follows:%water to add5 saturation2 7.0(2)1This test method is under the jurisdiction of ASTM Committee D07 on Woodand is the direct responsibility of Subcommittee D07.06 on Treatments for WoodProducts.Current edition approved March 1,2008.Published April 2008.Originallyapproved in 1974.Last previous edition approved in 1999 as D3345 74(1999).DOI:10.1520/D3345-08.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3Available from the American Wood Protection Association(AWPA),PO Box361784,Birmingham,AL 35236-1784,http:/.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 5.1.4 For example,the saturation point was reached at 20mL of water:Saturation520/100!31005 20.0%(3)%Water to add5 20.02 7.05 13.0%6.Collection of Termites6.1 Subterranean Termites,for example,(Reticulitermes,Coptotermes,spp.)Collect from a large colony(over50 000)in a natural forest situation,for example,from fallenlogs,stumps,and so forth,or from strong laboratory cultures.6.1.1 Remove short log sections to the laboratory and splitthem.Shake the insects out onto a tray or trays.Afterdistributing the debris and insects evenly on the tray(s),laydamp paper towels,sheets of kraft paper,and so forth,over thedebris.The termites will cling to the damp paper after a fewminutes.6.1.2 Prepare an 8 to 11-L(2 to 3-gal)pail by placing aboutten unfolded,slightly crumpled,damp paper towels in thebottom of the pail.Rinse these towels in distilled water andsqueeze damp a number of times.Cover these towels withabout ten unfolded,dry paper towels.6.1.3 Shake the damp towels covering the tray debris intothe above de