Designation:D4200−82(Reapproved2019)StandardTestMethodforEvaluatingInhibitoryEffectsofInkGridsonMembraneFilters1ThisstandardisissuedunderthefixeddesignationD4200;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethoddescribesaprocedurewherebytheuserofink-griddedmembranefiltersinwaterqualitystudiescanascertainwhetherornotthegridlinesaretoxicandinhibitorytobacterialgrowthwhenthemembraneanditsentrappedbacteriaareincubatedonasuitablemedia.1.2ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafety,health,andenvironmentalpracticesanddeter-minetheapplicabilityofregulatorylimitationspriortouse.1.4Thisinternationalstandardwasdevelopedinaccor-dancewithinternationallyrecognizedprinciplesonstandard-izationestablishedintheDecisiononPrinciplesfortheDevelopmentofInternationalStandards,GuidesandRecom-mendationsissuedbytheWorldTradeOrganizationTechnicalBarrierstoTrade(TBT)Committee.2.ReferencedDocuments2.1ASTMStandards:2D1129TerminologyRelatingtoWaterD1193SpecificationforReagentWater3.Terminology3.1Definitions:3.1.1Fordefinitionsoftermsusedinthisstandard,refertoTerminologyD1129.4.SummaryofTestMethod4.1Aheavybacterialsuspensionisfilteredthroughagriddedmembranefilter.Thebacterialconcentrationemployedissufficienttocovermuchofthemembranewithbacterialcolonies.4.2Afterfiltrationthemembraneisincubatedonasuitablemediumandthedistributionofthecoloniesandshapeofthecoloniesnotedintheareaaroundeachgridline.5.SignificanceandUse5.1Thistestmethodmaybeappliedtodeterminethesuitabilityofgrid-markedmembranefiltersforuseinbacte-riologicalculturetechniquesforthedetectionande...
