Designation:D4299-834m-70AMERICANSOCIETYFORTESTINGANDMATERIALS1916RaceSt.,Philadelphia,Pa.19103ReprintedfromtheAnnualBookofASTMStandards,CopyrightASTMIfnotlistedinthecurrentcombinedindex,willappearinthenextedition.StandardTestMethodsfarEFFECTOFBACTERIALCONTAMINATIONONPERMANENCEOFADHESIVEPREPARATIONSANDADHESIVESFILMS’ThisstandardisissuedunderthefwddesignationD4299;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,intheoseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(e)indicatesaneditorialchangesincethelastrevisionorreapproval.1.scope1.1Thesetestmethodscoverthedetermina-tionoftheeffectofbacterialcontaminationonthepermanencyof:1.1.1AdhesivePreparations,bydeterminingthecomparativeviscositiesandobservingtheappearanceoftheuncontaminatedandthecon-taminatedadhesiveswhentestedunderdefinedconditions,and1.1.2AdhesiveFilms,.byexposuretoculturesofselectedspeciesofbacteriagrowingonagarplates.1.2Thetestmethodsarenotappropriateforalladhesives.Thepresenceofcertainbiocidesmightyieldfalsenegativetestsasaresultofirreversiblereactionwiththemediumconstitu-ents.Also,theefficacyofsomebiocidesmaychangeinstorageduetothechemicalandther-malenvironmenttowhichtheyaresubjectedascomponentsofcertainadhesives.Thesetestmethodsalsoarenotdesignedtocoverthepos-sibilityofwatersolublebiocidesleachingoutofthegiueline,sotheyarenotappropriatefordeterminingtheeffectofmoldcontaminationonadhesivesunderwater-soakingconditions.1.3Thisstandardmayinvolvehazardousma-terials,operations,andequipment.Thisstandarddoesnotpurporttoaddressallofthesafityprob-lemsassociatedwithitsuse.Itistheresponsibil-ityofwhoeverusesthisstandardtoconsultandestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimi-tationspriortouse.Specificprecautionarystate-mentsaregiveninSection7.2.ApplicableDocuments2.1ASTMStandards:DI174TestMethodforEffectofBacterialContaminationonPermanenceofAdhesivePreparationsandAdhesiveBonds’...
