Designation:D5392−19StandardTestMethodforIsolationandEnumerationofEscherichiacoliinWaterbytheTwo-StepMembraneFilterProcedure1ThisstandardisissuedunderthefixeddesignationD5392;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethoddescribesamembranefilter(MF)procedureforthedetectionandenumerationofEscherichiacoli,abacteriumfoundexclusivelyinthefecesofhumansandotherwarm-bloodedanimals.Thepresenceofthesemicroor-ganismsinwaterisanindicationoffecalpollutionandthepossiblepresenceofentericpathogens.Thesebacteriaarefoundinwaterandwastewaterinawiderangeofdensities.Thedetectionlimitofthisprocedureisonecolonyformingunit(CFU)pervolumefiltered.1.2Thistestmethodhasbeenusedsuccessfullywithtemperatefreshandmarineambientwaters,andwastewaters.Itistheuser’sresponsibilitytoensurethevalidityofthistestmethodforwatersofothertypes.1.3ThevaluesstatedinSIunitsaretoberegardedasstandard.1.4Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafety,health,andenvironmentalpracticesanddeter-minetheapplicabilityofregulatorylimitationspriortouse.Forspecifichazardstatements,seeSection9.1.5Thisinternationalstandardwasdevelopedinaccor-dancewithinternationallyrecognizedprinciplesonstandard-izationestablishedintheDecisiononPrinciplesfortheDevelopmentofInternationalStandards,GuidesandRecom-mendationsissuedbytheWorldTradeOrganizationTechnicalBarrierstoTrade(TBT)Committee.2.ReferencedDocuments2.1ASTMStandards:2D1129TerminologyRelatingtoWaterD1193SpecificationforReagentWaterD3370PracticesforSamplingWaterfromFlowingProcessStreamsD3870PracticeforEstablishingPerformanceCharacteristicsforColonyCountingMethodsinMicrobiology(With-drawn2000)3D5465PracticesforDeterminingMicrobialColonyCount...
