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ASTM_E_2720_-_10.pdf
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TM_E_2720_ _10
Designation:E272010Standard Test Method forEvaluation of Effectiveness of Decontamination Proceduresfor Air-Permeable Materials when Challenged with BiologicalAerosols Containing Human Pathogenic Viruses1This standard is issued under the fixed designation E2720;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.INTRODUCTIONMany communicable diseases are often spread through the aerosol route of exposure.The dropletnuclei formed in these aerosols may infect susceptible individuals directly or contaminate environ-mental surfaces and render them fomites for further spread of disease.The characteristics of thedroplet nuclei(particle size and composition)will influence the viability of microorganisms whenexposed to environmental stresses but may also shield them from physical and chemical decontami-nants.The wide variations in the types and levels of such protective/shielding ingredients can haveimpact on the effectiveness of surface decontaminants.This test method is designed to simulate thedeposition of droplet nuclei from human respiratory secretions onto and into air-permeablemembranes.It is primarily focused on influenza viruses but other respiratory viruses or surrogateviruses could be used.Protocols for assessing the microbicidal activity of disinfectants are alsodescribed.1.Scope1.1 This test method is designed to evaluate decontamina-tion methods(physical,chemical,self-decontaminating mate-rials)when used on air-permeable materials contaminated withvirus-containing droplet nuclei.1.2 This test method defines the conditions for simulatingrespiratory droplet nuclei produced by humans.1.3 The method is specific to influenza viruses but could beadapted for work with other types of respiratory viruses orsurrogates(Appendix X6).1.4 This test method is suitable only for air-permeablematerials.1.5 This test method does not address the performance ofdecontaminants against microbes expelled via blood splatter,vomit,or fecal contamination.1.6 This test method should be performed only by thosetrained in bioaerosols,microbiology,or virology,or combina-tions thereof.1.7 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.8 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2E1052 Test Method to Assess the Activity of Microbicidesagainst Viruses in SuspensionE2197 Quantitative Disk Carrier Test Method for Determin-ing Bactericidal,Virucidal,Fungicidal,Mycobactericidal,and Sporicidal Activities of ChemicalsE2721 Test Method for Evaluation of Effectiveness of De-contamination Procedures for Surfaces When Challengedwith Droplets Containing Human Pathogenic Viruses1This test method is under the jurisdiction of ASTM Committee E35 onPesticides,Antimicrobials,and Alternative Control Agents and is the directresponsibility of Subcommittee E35.15 on Antimicrobial Agents.Current edition approved Oct.1,2010.Published February 2011.DOI:10.1520/E272010.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 2.2 IEST Standards:IEST-RP-CC003.3Garment System Considerations forClean Rooms and Other Controlled Environments32.3 Department of Defense Standards:CA06PRO411 Method for Evaluating Air Purification Tech-nologies for Collective Protections Using Viable Micro-bial Aerosols42.4 EPA Standards:EPA 6004-84013(N16)USEPA Manual of Methods forVirology52.5 WHO Standards:WHO Manual on Animal Influenza Diagnosis and Surveil-lance63.Terminology3.1 Definitions:3.1.1 aerosol,na suspension of solid or liquid particles ina gas medium.3.1.2 air-permeable material,nno standard definition isavailable;for the purpose of this test method,air-permeablematerial is described as any membrane that has a pressure drop twice that of high efficiency particulate air(HEPA)media inthe same test environment.3.1.3 biological aerosol,naerosol comprising particles ofbiological origin or activity which may affect living thingsthrough infectivity,allergencity,toxicity,or pharmacologicaland other processes.3.1.4 influenza,nan infectious disease of birds and mam-mals caused by RNA viruses of the family Orthomyxoviridae.3.1.5 protective factor,nsoluble or insoluble materialco-deposited with microorganisms that directly

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