Designation:D5196−06(Reapproved2018)StandardGuideforBio-ApplicationsGradeWater1ThisstandardisissuedunderthefixeddesignationD5196;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thisguideisintendedtodescribethechemicalandbiologicalcharacteristicsofwatertobeusedwhenevercriticalpurityisessentialtotheuseintendedinlaboratorybio-applications,forexample,clinical,pharmaceutical,andbio-medical.Theimportanceofsuchareagentisoftenunderesti-mateddespitetheimpactthatitcanhave.1.2Thisguideisnotintendedtobeusedasareferenceinpreparingwaterforinjectables.Generally,theappropriateuseofthisguidemayincludeexperimentsinvolvingtissueculture,chromatography,massspectrometry,polymerasechainreac-tion(PCR),deoxyribonucleicacid(DNA)sequencing,DNAhybridization,electrophoresis,molecularbiologyoranalyseswheremolecularconcentrationsofimpuritiesmaybeimpor-tant.1.3ForalltheotherapplicationslinkedtoanASTMmethodandnotbio-sensitivethatrequirepurifiedwater,itisrecom-mendedthatSpecificationD1193orGuideD5127becon-sulted.1.4ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.5Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafety,health,andenvironmentalpracticesanddeter-minetheapplicabilityofregulatorylimitationspriortouse.1.6Thisinternationalstandardwasdevelopedinaccor-dancewithinternationallyrecognizedprinciplesonstandard-izationestablishedintheDecisiononPrinciplesfortheDevelopmentofInternationalStandards,GuidesandRecom-mendationsissuedbytheWorldTradeOrganizationTechnicalBarrierstoTrade(TBT)Committee.2.ReferencedDocuments2.1ASTMStandards:2D1125TestMethodsforElectricalConductivityandResis-tivityofWaterD1129TerminologyRelatingtoWaterD119...
