Designation:D4994−19StandardPracticeforRecoveryofVirusesfromWastewaterSludges1ThisstandardisissuedunderthefixeddesignationD4994;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope*1.1Thispracticeisusedfortherecoveryofvirusesfromwastewatersludgesandfavorstheenteroviruses.1.2Bothproceduresareapplicabletoraw,digested,anddewateredsludges.SectionsProcedureA—Adsorption7to10ProcedureB—Sonication11to151.3Thispracticewastestedonstandardizedsludgesasdescribedin10.1.Itistheuser’sresponsibilitytoensurethevalidityofthispracticeforuntestedmatrices.1.4ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.5Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafety,health,andenvironmentalpracticesanddeter-minetheapplicabilityofregulatorylimitationspriortouse.1.6OnlyadequatelytrainedpersonnelshouldbeallowedtoperformtheseproceduresandshouldusesafetyprecautionsrecommendedbytheU.S.PublicHealthService,CenterforDiseaseControl,2forworkwithpotentiallyhazardousbiologi-calorganisms.1.7Thisinternationalstandardwasdevelopedinaccor-dancewithinternationallyrecognizedprinciplesonstandard-izationestablishedintheDecisiononPrinciplesfortheDevelopmentofInternationalStandards,GuidesandRecom-mendationsissuedbytheWorldTradeOrganizationTechnicalBarrierstoTrade(TBT)Committee.2.ReferencedDocuments2.1ASTMStandards:3D1129TerminologyRelatingtoWaterD1193SpecificationforReagentWater3.Terminology3.1Definitions:3.1.1Fordefinitionsoftermsusedinthisstandard,refertoTerminologyD1129.4.SignificanceandUse4.1Althoughmanylaboratoriesarepresentlyisolatingvi-rusesfromsludge,avalidcomparisonofdatageneratedhasnotbeenpossiblebecauseofthelackofastandardtestmethod(s)...
