ASTM_D_5245_-_92_2012.pdf

Designation:D524592(Reapproved 2012)Standard Practice forCleaning Laboratory Glassware,Plasticware,and EquipmentUsed in Microbiological Analyses1,2This standard is issued under the fixed designation D5245;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 In microbiology,clean glassware is crucial to ensurevalid results.Previously used or new glassware must bethoroughly cleaned.Laboratory ware and equipment that arenot chemically clean are responsible for considerable losses inpersonnel time and supplies in many laboratories.These lossesmay occur as down time when experiments clearly have beenadversely affected and as invalid data that are often attributedto experimental error.Chemical contaminants that adverselyaffect experimental results are not always easily detected.Thispractice describes the procedures for producing chemicallyclean glassware.1.2 The values stated in SI units are to be regarded as thestandard.1.3 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.For specificprecautions,see Section 5,7.3.1,and Note 1and Note 2.2.Referenced Documents2.1 ASTM Standards:3D1193 Specification for Reagent Water3.Significance and Use3.1 This practice provides uniform guidance for cleaningthe laboratory glassware,plasticware,and equipment used inroutine microbiological analyses.However,tests that are ex-tremely sensitive to toxic agents(such as virus assays)mayrequire more stringent cleaning practices.24.Reagents4.1 Purity of ReagentsReagent grade chemicals shall beused in all tests.Unless otherwise indicated,it is intended thatall reagents conform to the specifications of the Committee onAnalytical Reagents of the American Chemical Society wheresuch specifications are available.4Other grades may be used,provided it is first ascertained that the reagent is of sufficientlyhigh purity to permit its use without lessening the accuracy ofthe determination.4.2 Purity of Water Unless otherwise indicated,referencesto water shall be understood to mean Type IV of SpecificationD1193.4.3 Detergent Solution,for machine-washing glassware andequipment.Use according to manufacturers instructions.4.4 Detergent Powder,for hand-washing glassware andequipment.Use them according to manufacturers instructions.There now are a number of effective biogradable detergentproducts available that allow the laboratory to avoid acidcleaning of most if not all glassware.4.5 Nitric Acid(1+9)Pour 100 mL of concentratedHNO3slowly into 900 mL of water.To avoid dangeroussplatters,never pour water into concentrated acid.4.6 Chromic Acid SolutionChromic acid replacement5isapplicable.5.Hazards5.1 The analyst/technician must know and observe normalgood laboratory practices and safety procedures required in amicrobiology laboratory in preparing,using,and disposing of1This practice is under the jurisdiction of ASTM Committee D19 on Water andis the direct responsibility of Subcommittee D19.24 on Water Microbiology.Current edition approved June 1,2012.Published August 2012.Originallyapproved in 1992.Last previous edition approved in 2005 as D5245 92(2005).DOI:10.1520/D5245-92R12.2A significant portion of this practice was taken from:Berg,G.,Safferman,R.S.,Dahling,D.R.,Berman,D.,and Hurst,C.J.,USEPA Manual of Methods forVirology,EPA-600/4-84-013,Chapt.2,“Cleansing Laboratory Ware andEquipment,Environmental Monitoring and Support LaboratoryCincinnati,”USEPA,Cincinnati,OH.3For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.4“Reagent Chemicals,American Chemical Society Specifications,Am.Chemi-cal Soc.,Washington,DC.For suggestions on the testing of reagents not listed bythe American Chemical Society,see“Analar Standards for Laboratory Chemicals,”BDH Ltd.Poole Dorset,U.K.and the“United States Pharmacopeia.”5The sole source of supply of the apparatus known to the committee at this timeis Monostat Corp.,519 Eighth St.,New York,NY 10018.If you are aware ofalternative suppliers,please provide this information to ASTM InternationalHeadquarters.Your comments will receive careful consideration at a meeting of theresponsible technical committee,1which you may attend.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 cultures,reagents,and materials,and while operating steriliza-tion and other equipment and instrumentation.5.2 Sterilize contaminated laboratory war