ASTM_D_4478_-_85_scan.pdf

t Designation:D 4478-85 Reprinted from the Annual Book of ASiM Standards,Copynght ASTM If not listed i!lfhe current combined index,will appear in the next edition.Standard Test Methods for OXYGEN UPTAKE1 This standard is issued under the fixed designation D 4478;the number immediately following the designation indicates the year of original adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.A superscript epsilon(-1 a/)0 STAND AND CLAMP MAGNETIC 1500 ml STIRRING BAR-BEAKER FOAM INSULATION-MAGNETIC STIRRER.-DIS:OLVED OXYGEN PROBE RECORDER FIG.1 Apparatus for Determination of Oxygen Uptake N2 TO DEAERATE EQUILIBRIUM DO rFIGURE 3.,.0o:I!V=EI!I!RL,.AY-,/SAMPLE 02 RECOVERY CURVE?-NITROGEN CONTROL,Oz RECOVERY CURVE QL-REAERA_TIONBYSTIRRING-TIME-FIG.2 Nitroaen Control r5 I EQUILIBRIUM DO!1/SAMPLE ADDED w IMMEDIATE:i OXYGEN UPTAKE _!_-sAMPLE Oz RECOVERY CURVE EQUILIBRIUM DO 0 OL-TIME-FIG.3 Biodegradable Compound or Wastewater(Typical Curve for Method B)9%Th.O.D.34 32 33 I 33 z w!0 Ill t 04478 NOTE VERY SLOW RECOVERY o-=TIME-FIG.4 Bioddahle Comround or WastewPter(Typi(lll Curve for Method A)z EQUILIBRIUM DO UJ lojr-S-A_M_P_L_EAT0-0-EO-IIl NOTE VERY SLOW DECREASE IN DO Ill i5 0-z U.J C)TIME-FIG.5 Slowly Biodegradable Material or Unacclimated Sludge EQUILIBRIUM DO SAMPLE ADDED-o NOTE:NO DECREASE IN DO Ill Ill i5 0-TIME-FIG.6 Non-Biuddable Substance EQUILIBRIUM DO SATURATION DO 1 0 In In Ci 0k-TIME-FIG.7 Inhibitory or Toxic Substance Concentration 10 4t 04478 z w(!)g I Ill Ill 0 SAMPLE ADDED SAMPLE RECOVERY CURVE oL-TIME-NoTE-Large sample.nay be inhibitory or toxic;smaller sample may be biodegradable,but response may be too small to be detected.FIG.8 SUghtly Inhibitory Substance ANNEX(Mandatory Information)AI.TYPICAL EXPERIMENT AND DATA HANDLING A 1.1.Sludge-Phenol-acclimated activated sludge;200 mL of settled sludge diluted to 1.0 L.A 1.2 Sample Tested-1.0 g/L phenol solution;used 3.0 mL in test.L A1.3 Oxygen Uptake Test Conditions:Phenol-acclimated sludge=200 mL settled sludge/pH=6.9 Equilibrium 00=4.90 Temperature=22 to 23C 00 scale=0 to 10 mg Recorder chart speed=30 in./h Tap water,equivalent to Specification D 1193,Type IV,was used for required washing and final dilution of the phenol-acclimated activated sludge.Al.4 Nitrogen Control:Time,min OO,mg/L 00 0 1.03 I 1.30 0.27 2 1.57 0.27 3 1.82 0.25 4 2.07 0.25 5 2.29 0.22 6 2.48 0.19 1 2.66 0.18 8 2.83 0.17 9 3.00 0.17 10 3.19 0.19 II 3.32 0.13 12 3.46 0.14 13 3.54 0.08 14 3.64 0.10 15 3.74 0.10 16 3.84 0.10 17 3.93 0.09 18 4.01 0.08 19 4.10 0.09 20 4.18 0.08 21 4.25 0.07 22 4.30 0.05 11 Time,min 23 00,mg/L 4.34 aoo 0.04 Al.5 Sample Data:Equilibrium 00=4.90 Added 3.0 mL of 1.0 g/L phenol solution Immediate Oxygen Uptake Recovery Time,DO,Time,00,Time,DO,min mg/L min mgfL min mg/L 0 I 2 3 4 5 4.90 3.00 2.40 0 I 2 3 4 5 6 7 8 9 10 11 12 13 14 15 2.40 2.48 2.60 2.71 2.88 3.00 3.12 3.22 3.32 3.42 3.51 3.60 3.68 3.75 3.83 3.92 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 3.99 4.03 4.09 4.14 4.19 4.22 4.25 4.29 4.30 4.31 4.40 A 1.5.1 Immediate Oxygen Depletion(IOD):Initial 00-lowest 00=IOD 4.90-2.40=2.50=IOD(Oxygen depletion,%=2.50/4.90 x 100=51%).1.5.2 Immediate Oxygen Uptake Coection(IC):4.90 3.00 10.30/3=3.43=avg 00 2.40 Tif.30 Then,refer to N2 control data;02 uptake rate is about 0.13 mg 02/min at a 00 of 3.43.IC=0.13 mg 02/min x 20 min=0.26 mg 02 Al.5.3 Immediate Oxygen Uptake(IOU):IOU,mg 02/mg phenol=IOD+IC/mg phenol=2.50+0.26/3.0=2.76/3.0=0.92 Theoretical oxygen demand for I mg phenol=2.38 mg02 0.92/2.38 x 100=39%of theoretical oxygen demand Al.5.4 Recovery Oxygen Uptake(ROU)(see Al.4 and Al.5):Sample Data Nitrogen Control Differ-Time,(aDO at ence min DO as Sample(aN-as)DO)aN 0 2.40 I 2.48 0.08 0.19 0.11 2 2.60 0.12 0.18 0.06 3 2.71 0.11 0.18 0.07 4 2.88 0.17 0.17 0 04478 Time,min Sample Data DO 3.00 as 0.12 Nitrogen Control(aDO at Sample DO)aN 0.17 Differ-ence(aN-as)Total mg 0,=0.11 ROU A Cut off at first difference of 0.20 or less.A.l.5.5 Summary:Sample=3.0 mL of 1.0 g/L phenol 3.0 mg phenol IOU=IOD+IC=2.50+0.26=2.76 mg 02 ROU=0.11 mg 02 Total oxygen=2.87 mg 02/L at 22 to uptake 23C Then,2.87 mg 02/3.0 mg phenol=0.96 mg 02/mg phenol Theoretical oxygen demand for 1.0 mg phenol=2.38 mg02 Then,0.96/2.38 x 100=40%of theoretical oxygen demand APPENDIX(Nonmandatory Information)X!.SUC-GESTED PROCEDURE FOR PRODUCTION OF ACCLIMATED ACTIVATED SLUDGE X 1.1 Many organic chemicals,alone or as compo-nents of an industrial wastewater,are not degraded by the microorganisms present in activated sludge derived from domestic sewage.If an acclimation period is pro-vided,however,quite often the activated sludge can develop the capability of degrading the foreign or-ganic chemicals.X 1.2 Prepare the following nutrient solution:Nutrient broth 40 g U=4g Potassium phosphate,dibasic 3 g Dissolve in tap water and make up to I L.Refrigerate.X 1.3 Collect 4 L of fresh activated sludge.NoTE XI.I-The procedure in Xl.3.1 to X