Designation:D5245−19StandardPracticeforCleaningLaboratoryGlassware,Plasticware,andEquipmentUsedinMicrobiologicalAnalyses1,2ThisstandardisissuedunderthefixeddesignationD5245;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope*1.1Inmicrobiology,cleanglasswareiscrucialtoensurevalidresults.Previouslyusedornewglasswaremustbethoroughlycleaned.Laboratorywareandequipmentthatarenotchemicallycleanareresponsibleforconsiderablelossesinpersonneltimeandsuppliesinmanylaboratories.Theselossesmayoccurasdowntimewhenexperimentsclearlyhavebeenadverselyaffectedandasinvaliddatathatareoftenattributedtoexperimentalerror.Chemicalcontaminantsthatadverselyaffectexperimentalresultsarenotalwayseasilydetected.Thispracticedescribestheproceduresforproducingchemicallycleanglassware.1.2ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafety,health,andenvironmentalpracticesanddeter-minetheapplicabilityofregulatorylimitationspriortouse.Forspecificprecautions,seeSection6,5.7.3.1,and8.3.1.1.4Thisinternationalstandardwasdevelopedinaccor-dancewithinternationallyrecognizedprinciplesonstandard-izationestablishedintheDecisiononPrinciplesfortheDevelopmentofInternationalStandards,GuidesandRecom-mendationsissuedbytheWorldTradeOrganizationTechnicalBarrierstoTrade(TBT)Committee.2.ReferencedDocuments2.1ASTMStandards:3D1129TerminologyRelatingtoWaterD1193SpecificationforReagentWater3.Terminology3.1Definitions:3.1.1Fordefinitionsoftermsusedinthisstandard,refertoTerminologyD1129.4.SignificanceandUse4.1Thispracticeprovidesuniformguidanceforcleaningthelaboratoryglassware,plasticware,andequipmentusedinrou...
