ASTM_D_5245_-_19.pdf

Designation:D524519Standard Practice forCleaning Laboratory Glassware,Plasticware,and EquipmentUsed in Microbiological Analyses1,2This standard is issued under the fixed designation D5245;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope*1.1 In microbiology,clean glassware is crucial to ensurevalid results.Previously used or new glassware must bethoroughly cleaned.Laboratory ware and equipment that arenot chemically clean are responsible for considerable losses inpersonnel time and supplies in many laboratories.These lossesmay occur as down time when experiments clearly have beenadversely affected and as invalid data that are often attributedto experimental error.Chemical contaminants that adverselyaffect experimental results are not always easily detected.Thispractice describes the procedures for producing chemicallyclean glassware.1.2 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.3 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.For specific precautions,see Section 6,5.7.3.1,and 8.3.1.1.4 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:3D1129 Terminology Relating to WaterD1193 Specification for Reagent Water3.Terminology3.1 Definitions:3.1.1 For definitions of terms used in this standard,refer toTerminology D1129.4.Significance and Use4.1 This practice provides uniform guidance for cleaningthe laboratory glassware,plasticware,and equipment used inroutine microbiological analyses.However,tests that are ex-tremely sensitive to toxic agents(such as virus assays)mayrequire more stringent cleaning practices.25.Reagents5.1 Purity of ReagentsReagent grade chemicals shall beused in all tests.Unless otherwise indicated,it is intended thatall reagents conform to the specifications of the Committee onAnalytical Reagents of the American Chemical Society wheresuch specifications are available.4Other grades may be used,provided it is first ascertained that the reagent is of sufficientlyhigh purity to permit its use without lessening the accuracy ofthe determination.1This practice is under the jurisdiction of ASTM Committee D19 on Water andis the direct responsibility of Subcommittee D19.24 on Water Microbiology.Current edition approved April 1,2019.Published April 2019.Originallyapproved in 1992.Last previous edition approved in 2012 as D5245 92(2012).DOI:10.1520/D5245-19.2A significant portion of this practice was taken from:Berg,G.,Safferman,R.S.,Dahling,D.R.,Berman,D.,and Hurst,C.J.,USEPA Manual of Methods forVirology,EPA-600/4-84-013,Chapter 2,“Cleansing Laboratory Ware andEquipment,Environmental Monitoring and Support LaboratoryCincinnati,”USEPA,Cincinnati,OH.3For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.4Reagent Chemicals,American Chemical Society Specifications,AmericanChemical Society,Washington,DC.For suggestions on the testing of reagents notlisted by the American Chemical Society,see Analar Standards for LaboratoryChemicals,BDH Ltd.,Poole,Dorset,U.K.,and the United States Pharmacopeiaand National Formulary,U.S.Pharmacopeial Convention,Inc.(USPC),Rockville,MD.*A Summary of Changes section appears at the end of this standardCopyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.1 5.2 Purity of WaterUnless otherwise indicated,referencesto water shall be understood to mean Type IV of SpecificationD1193.5.3 Bromothymol blue,0.4%.5.3.1 Bromothymol blue.5.3.2 Sodium hydroxide solution,240 g/L.5.3.3 Adding 16 mL of a NaOH solution(240 g/L)to 0.1 gof bromothymol blue.5.3.4 Dilute to 250 mL with Type III reagent water ofSpecification D1193.5.4 Detergent Solution,for machine-washing glassware andequipment.Use acc