Designation:D5246−19StandardTestMethodforIsolationandEnumerationofPseudomonasaeruginosafromWater1ThisstandardisissuedunderthefixeddesignationD5246;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope*1.1ThetestmethodcoverstheisolationandenumerationofPseudomonasaeruginosa.Testingwasperformedonspikedsamplesusingreagentgradewaterasthediluentfromsurfacewaters;recreationalwaters;groundwater,watersupplies;especiallyruralnonchlorinatedsources;wastewater;andsalinewaters.Thedetectionlimitofthistestmethodisonemicroorganismper100mL.1.2Thistestmethodwasusedsuccessfullywithreagentwater.Itistheuser’sresponsibilitytoensurethevalidityofthistestmethodforsurfacewaters,recreationalwaters,groundwater,ruralnonchlorinatedsources;wastewater;andsalinewaters.1.3ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.4Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafety,health,andenvironmentalpracticesanddeter-minetheapplicabilityofregulatorylimitationspriortouse.SpecifichazardstatementsaregiveninSection10.1.5Thisinternationalstandardwasdevelopedinaccor-dancewithinternationallyrecognizedprinciplesonstandard-izationestablishedintheDecisiononPrinciplesfortheDevelopmentofInternationalStandards,GuidesandRecom-mendationsissuedbytheWorldTradeOrganizationTechnicalBarrierstoTrade(TBT)Committee.2.ReferencedDocuments2.1ASTMStandards:2D1129TerminologyRelatingtoWaterD1193SpecificationforReagentWaterD2777PracticeforDeterminationofPrecisionandBiasofApplicableTestMethodsofCommitteeD19onWaterD3370PracticesforSamplingWaterfromFlowingProcessStreams3.Terminology3.1Definitions:3.1.1Fordefinitionsoftermsusedinthisstandard,refertoTerminologyD1129.3.2...
