ASTM_D_5075_-_01_2012e1.pdf

Designation:D507501(Reapproved 2012)1Standard Test Method forNicotine and 3-Ethenylpyridine in Indoor Air1This standard is issued under the fixed designation D5075;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1NOTEReapproved with editorial change to 1.5 in April 2012.1.Scope1.1 This test method covers the sampling/analysis of nico-tine and 3-ethenylpyridine(3-EP)in indoor air.This testmethod is based upon the collection of nicotine and 3-EP byadsorption on a sorbent resin,extraction of nicotine and 3-EPfrom the sorbent resin,and determination by gas chromatog-raphy(GC)with nitrogen selective detection.(1)21.2 The active samplers consist of an XAD-4 sorbent tubeattached to a sampling pump.This test method is applicable topersonal or area sampling.1.3 This test method is limited in sample duration by thecapacity of the XAD-4 tube for nicotine(about 300 g).Thistest method has been evaluated up to 24-h sample duration;however,samples are typically acquired for at least 1 h(sometimes only 1 h).(2)1.4 For this test method,limits of detection(LOD)andquantitation(LOQ)for nicotine at a sampling rate of 1.5 L/minare,respectively,0.11 g/m3and 0.37 g/m3for 1-h sampleduration and 0.01 g/m3and 0.05 g/m3for 8-h sampleduration.The LOD and LOQ for 3-EP at a sampling rate of 1.5L/min are,respectively,0.06 g/m3and 0.19 g/m3for 1-hsample duration and 0.01 g/m3and 0.02 g/m3for 8-h sampleduration(2).Both LOD and LOQ can be reduced by increasingthe sensitivity of the thermionic specific detector.1.5 UnitsThe values stated in SI units are to be regardedas standard.No other units of measurement are included in thisstandard.1.6 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.Specific precau-tionary information is given in 13.6.2.Referenced Documents2.1 ASTM Standards:3D1356 Terminology Relating to Sampling and Analysis ofAtmospheresD1357 Practice for Planning the Sampling of the AmbientAtmosphereD3631 Test Methods for Measuring Surface AtmosphericPressureD5337 Practice for Flow Rate Adjustment of Personal Sam-pling PumpsE260 Practice for Packed Column Gas ChromatographyE355 Practice for Gas Chromatography Terms and Relation-ships3.Terminology3.1 DefinitionsFor definitions of terms used in this testmethod,refer to Terminology D1356 and Practice E355.3.2 Definitions of Terms Specific to This Standard:3.2.1 environmental tobacco smoke(ETS)an aged,dilutecomposite of exhaled tobacco smoke and smoke from tobaccoproducts.3.2.2 nitrogen-phosphorus detector(NPD)a highly sensi-tive device selective for detection of nitrogen-and phosphorus-containing organic compounds.3.2.3 XAD-4resinmacroreticularpolystyrene-divinylbenzene copolymer beads.4.Summary of Test Method4.1 A known volume of air is drawn through a sorbentsampling tube containing XAD-4 resin to adsorb the nicotineand 3-EP present.4.2 The XAD-4 sorbent tube contents are transferred to a2-mL autosampler vial,and the nicotine and 3-EP are desorbedwith ethyl acetate containing 0.01%triethylamine and aknown quantity of quinoline,the internal standard.1This test method is under the jurisdiction of ASTM Committee D22 on AirQuality and is the direct responsibility of Subcommittee D22.05 on Indoor Air.Current edition approved April 1,2012.Published July 2012.Originallyapproved in 1990.Last previous edition approved in 2007 as D5075-01(2007).DOI:10.1520/D5075-01R12E01.2The boldface numbers in parentheses refer to a list of references at the end ofthe text.3For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 4.3 An aliquot of the desorbed sample is injected into a gaschromatograph equipped with a thermionic-specific(nitrogen-phosphorus)detector.4.4 The areas of the resulting nicotine and 3-EP peaks areeach divided by the area of the internal standard peak andcompared with area ratios obtained from the injection ofstandards.5.Significance and Use5.1 In order to estimate ETS concentrations,there needs tobe a marker or tracer for ETS that is unique or highly specificto tobacco smoke,in sufficient concentrations in air to bemeasured easily at realistic smoking rates,and in constantproportion to the other components of ETS for a variety oftobacco blends and environmental conditions.Nicotine and3-ethenylpyridine have been used as tracers of the va