Designation:D5660−96(Reapproved2009)StandardTestMethodforAssessingtheMicrobialDetoxificationofChemicallyContaminatedWaterandSoilUsingaToxicityTestwithaLuminescentMarineBacterium1ThisstandardisissuedunderthefixeddesignationD5660;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethod(1)2coversaprocedurefortherapidevaluationofthetoxicity3ofwastewatersandaqueousextractsfromcontaminatedsoilsandsediments,totheluminescentmarinebacteriumPhotobacteriumphosphoreum,4priortoandfollowingbiologicaltreatment.Thistestmethodismeantforuseasameanstoassesssamplesresultingfrombiotreatabilitystudies.SensitivitydataforP.phosphoreumtoover1300chemicalshavebeenreportedintheliterature(2).Someofthepublicationsareveryrelevanttothistestmethod(3).Thedataobtainedfromthistestmethod,whencombinedwithrespirometry,totalorganiccarbon(TOC),biochemicaloxygendemand(BOD),chemicaloxygendemand(COD),orspectro-photometricdata,canassistinthedeterminationofthedegreeofbiodegradabilityofacontaminantinwater,soil,orsediment(3).ThepercentagedifferencebetweentheIC20oftreatedanduntreatedsampleisusedtoassesstheprogressofdetoxifica-tion.1.2Thistestmethodisapplicabletotheevaluationofthetoxicity(toaspecificmicrobe)anditsimplicationonthebiodegradationofaqueoussamplesfromlaboratoryresearchbio-reactors(liquidorsoil),pilot-plantbiologicaltreatmentsystems,full-scalebiologicaltreatmentsystems,andlandapplicationprocesses(seeNotes1and2).NOTE1—Ifthebiologicallytreatedmaterialistobedischargedinsuchamannerastopotentiallyimpactsurfacewatersandgroundwater,orboth,thentheusermustconsultappropriateregulatoryguidancedocu-mentstodeterminethepropertestspeciesforevaluatingpotentialenvironmentalimpact(4).Correlationsbetweendataconcerningreductionintoxicityproducedbythistestmethodandbyproceduresforacuteorshort-termchr...
