ASTM_D_6081_-_22.pdf

Designation:D608122Standard Practice forAquatic Toxicity Testing of Lubricants:Sample Preparationand Results Interpretation1This standard is issued under the fixed designation D6081;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope*1.1 This practice covers procedures to be used in thepreparation of lubricants or their components for toxicitytesting in aquatic systems and in the interpretation of the resultsof such tests.1.2 This practice is suitable for use on fully-formulatedlubricants or their components that are not completely solubleat the intended test treat rates.It is also suitable for use withadditives,if the additive is tested after being blended into acarrier fluid at the approximate concentration as in the intendedfully formulated lubricant.The carrier fluid shall meet theabove solubility criterion,be known to be minimally toxic inthe toxicity test in which the material will be tested,and beknown to have a chemical composition similar to the rest of theintended fully formulated lubricant.1.3 Samples prepared in accordance with this practice maybe used in acute or chronic aquatic toxicity tests conducted infresh water or salt water with fish,large invertebrates,or algae.This practice does not address preparation of samples for planttoxicity testing other than algae.1.4 Standard acute and chronic aquatic toxicity proceduresare more appropriate for lubricants with compositions that arecompletely soluble at the intended test treat rates(1,2,3,4,5).21.5 This practice is intended for use with lubricants orlubricant components of any volatility.1.6 This practice does not address any questions regardingthe effects of any lubricant or lubricant component on humanhealth.1.7 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.8 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.1.9 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:3D1129 Terminology Relating to WaterD1193 Specification for Reagent WaterD4175 Terminology Relating to Petroleum Products,LiquidFuels,and LubricantsE729 Guide for Conducting Acute Toxicity Tests on TestMaterials with Fishes,Macroinvertebrates,and Amphib-iansE943 Terminology Relating to Biological Effects and Envi-ronmental Fate3.Terminology3.1 For definitions of terms used in this practice,seeTerminologies D1129,D4175,and E943.3.2 Definitions:3.2.1 acute toxicity test,na comparative test in which arepresentative subpopulation of organisms is exposed to dif-ferent treat rates of a test material and is observed for a shortperiod,usually not constituting a substantial portion of theirlife span.3.2.2 chronic toxicity test,na comparative test in which arepresentative subpopulation of organisms is exposed to dif-ferent treat rates of a test material and is observed for a periodof time that constitutes a major portion of their life span.1This practice is under the jurisdiction of ASTM Committee D02 on PetroleumProducts,Liquid Fuels,and Lubricants and is the direct responsibility of Subcom-mittee D02.12 on Environmental Standards for Lubricants.Current edition approved April 1,2022.Published April 2022.Originallyapproved in 1998.Last previous edition approved in 2020 as D6081 20.DOI:10.1520/D6081-22.2The boldface numbers in parentheses refer to the list of references at the end ofthis standard.3For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.*A Summary of Changes section appears at the end of this standardCopyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.13.2.3 ecotoxicity,npropensity of a test material to produceadverse behavioral,biochemical,or physiological effects innon-human organisms o