ASTM_D_5590_-_00_2010e1.pdf

Designation:D559000(Reapproved 2010)1Standard Test Method forDetermining the Resistance of Paint Films and RelatedCoatings to Fungal Defacement by Accelerated Four-WeekAgar Plate Assay1This standard is issued under the fixed designation D5590;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1NOTEAn editorial change was made in 7.5 in November 2012.1.Scope1.1 This test method covers an accelerated method fordetermining the relative resistance of two or more paints orcoating films to fungal growth.1.2 The values stated in SI units are to be regarded as thestandard.The values given in parentheses are for informationonly.1.3 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D822 Practice for Filtered Open-Flame Carbon-Arc Expo-sures of Paint and Related CoatingsD3273 Test Method for Resistance to Growth of Mold on theSurface of Interior Coatings in an Environmental Cham-berD3456 Practice for Determining by Exterior Exposure Teststhe Susceptibility of Paint Films to MicrobiologicalAttackD4141 Practice for Conducting Black Box and Solar Con-centrating Exposures of CoatingsD4587 Practice for Fluorescent UV-Condensation Expo-sures of Paint and Related CoatingsD5031 Practice for Enclosed Carbon-Arc Exposure Tests ofPaint and Related CoatingsG21 Practice for Determining Resistance of Synthetic Poly-meric Materials to Fungi3.Summary of Test Method3.1 This test method outlines a procedure to(1)prepare asuitable specimen for testing,(2)inoculate the specimen withthe proper fungal species,(3)expose the inoculated samplesunder the appropriate conditions for growth,and(4)provide aschedule and guidelines for visual growth ratings.This testmethod is not designed to include all the necessary proceduresto maintain the proper microbiological techniques required toprovide the most accurate results.4.Significance and Use4.1 Defacement of paint and coating films by fungal growth(mold,mildew)is a common phenomenon,and defacement byalgal growth can also occur under certain conditions.It isgenerally known that differences in the environment,lighting,temperature,humidity,substrate pH,and other factors inaddition to the coating composition affect the susceptibility ofa given painted surface.This test method attempts to provide ameans to comparatively evaluate different coating formulationsfor their relative performance under a given set of conditions.It does not imply that a coating that resists growth under theseconditions will necessarily resist growth in the actual applica-tion.NOTE1It is hoped that a ranking of relative performance would besimilar to that ranked from outdoor exposures.However,this test methodshould not be used as a replacement for exterior exposure(that is,PracticeD3456)since many other factors,only a few of which are listed will affectthose results.NOTE2Several companies have reported reasonable correlation ofresults from this test with actual use when testing film-forming,pigmentedcoatings.Round-robin testing of this test method versus exterior exposureis planned.4.2 Familiarity with microbiological techniques is required.This test method should not be used by persons without at leastbasic microbiological training.5.Apparatus and Materials5.1 Balance,capable of weighing to 0.10 g.1This test method is under the jurisdiction of ASTM Committee D01 on Paintand Related Coatings,Materials,and Applications and is the direct responsibility ofSubcommittee D01.28 on Biodeterioration.Current edition approved June 1,2010.Published July 2010.Originally approvedin 1994.Last previous edition approved in 2005 as D5590 00(2005).DOI:10.1520/D5590-00R10E01.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 5.2 Incubator,or other device capable of maintaining aconstant temperature between 25 and 30C,relative humidityof 85%.5.3 Refrigerator,or other device capable of maintaining atemperature of 4 6 2C.5.4 Petri Dishes,100 by 15 mm(3.9 by 0.6 in.).5.5 Autoclave,capable of producing 103 kPa(15 psi)ofsteam pressure at 121C and maintaining it for a minimum of15 min.An autoclave is not necessary if pre-prepared mediaplates are used.5.6 Paint Brush,coarse bristle,12 to 19 mm(12to34in.).5.7 Substrate,Filter Paper(Glass fiber,Grade 391,4.2 cm(1.65 in