ASTM_D_5412_-_93_2011e1.pdf

Designation:D541293(Reapproved 2011)1Standard Test Method forQuantification of Complex Polycyclic Aromatic HydrocarbonMixtures or Petroleum Oils in Water1This standard is issued under the fixed designation D5412;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1NOTEEditorial corrections were made throughout in March 2014.1.Scope1.1 This test method covers a means for quantifying orcharacterizing total polycyclic aromatic hydrocarbons(PAHs)by fluorescence spectroscopy(Fl)for waterborne samples.Thecharacterization step is for the purpose of finding an appropri-ate calibration standard with similiar emission and synchro-nous fluorescence spectra.1.2 This test method is applicable to PAHs resulting frompetroleum oils,fuel oils,creosotes,or industrial organicmixtures.Samples can be weathered or unweathered,but eitherthe same material or appropriately characterized site-specificPAH or petroleum oil calibration standards with similar fluo-rescence spectra should be chosen.The degree of spectralsimilarity needed will depend on the desired level of quantifi-cation and on the required data quality objectives.1.3 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.4 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D1129 Terminology Relating to WaterD1193 Specification for Reagent WaterD2777 Practice for Determination of Precision and Bias ofApplicable Test Methods of Committee D19 on WaterD3325 Practice for Preservation of Waterborne Oil SamplesD3326 Practice for Preparation of Samples for Identificationof Waterborne OilsD3415 Practice for Identification of Waterborne OilsD3650 Test Method for Comparison of Waterborne Petro-leum Oils By Fluorescence AnalysisD4489 Practices for Sampling of Waterborne OilsD4657 Test Method for PolynuclearAromatic Hydrocarbonsin Water(Withdrawn 2005)3E131 Terminology Relating to Molecular SpectroscopyE169 Practices for General Techniques of Ultraviolet-VisibleQuantitative AnalysisE275 Practice for Describing and Measuring Performance ofUltraviolet and Visible SpectrophotometersE388 Test Method for Wavelength Accuracy and SpectralBandwidth of Fluorescence SpectrometersE578 Test Method for Linearity of Fluorescence MeasuringSystemsE579 Test Method for Limit of Detection of Fluorescence ofQuinine Sulfate in Solution3.Terminology3.1 DefinitionsFor definitions of terms used in this testmethod,refer to Terminology D1129,Terminology E131,andPractice D3415.4.Summary of Test Method4.1 This test method consists of fluorescence analysis ofdilute solutions of PAHs or petroleum oils in appropriatesolvents(spectroquality solvents such as cyclohexane or otherappropriate solvents,for example,ethanol,depending onpolarity considerations of the sample).The test method re-quires an initial qualitative characterization step involving bothfluorescence emission and synchronous spectroscopy in orderto select appropriate calibration standards with similar fluores-cence spectra as compared to the samples(see Annex A1 forthe definition of spectral similarity).Intensities of peak1This test method is under the jurisdiction of ASTM Committee D19 on Waterand is the direct responsibility of Subcommittee D19.06 on Methods forAnalysis forOrganic Substances in Water.Current edition approved May 1,2011.Published June 2011.Originallyapproved in 1993.Last previous edition approved in 2005 as D5412 93(2005).DOI:10.1520/D5412-93R11E01.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3The last approved version of this historical standard is referenced onwww.astm.org.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 maxima of suitable emission spectra are then used to developcalibration curves for quantification.NOTE1Although some sections of the characterization part of this testmethod are similar to Test Method D3650,there are also significantdifferences(see Annex A1).Since the purpose and intent of the two testmethods are different,one should not be substituted for the other.5.Significance and Use5.1 This test method is useful for characterization and rapidquantification of PAH mixtures including petroleum oils,fuels,creosotes,and industrial organic mixtures,either waterborne orobtained from tanks.5.2 The unknown