ASTM_D_6271_-_10_2016.pdf

Designation:D627110(Reapproved 2016)Standard Test Method forEstimating Contribution of Environmental Tobacco Smoketo Respirable Suspended Particles Based on Solanesol1This standard is issued under the fixed designation D6271;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method covers the sampling/analysis of respi-rable suspended particles(RSP)and the estimation of the RSPfraction attributable to environmental tobacco smoke(ETS).The test method is based on collection of total RSP on amembrane filter,extraction of the filter in methanol,anddetermination of solanesol,a C45isoprenoid alcohol,by highperformance liquid chromatography(HPLC)with ultraviolet(UV)detection.1.2 This test method is compatible with the determinationsof gravimetric RSP,ultraviolet particulate matter(UVPM),andfluorescent particulate matter(FPM)(see Test MethodsD5955),but does not require them.UVPM and FPM,whichare based on the ultraviolet absorbance and fluorescence of thefilter extract,are also used to estimate the contribution of ETSto RSP.1.3 The sampling components consist of a 1.0-m pore sizepolytetrafluoroethylene(PTFE)membrane filter in a filtercassette connected on the inlet end to a particle size separatingdevice and,on the outlet end,to a sampling pump.This testmethod is applicable to personal and area sampling.1.4 This test method is limited in sample duration only bythe capacity of the membrane filter.The test method has beenevaluated up to 24-h sample duration;a minimum sampleduration of 1 h is recommended.1.5 Limits of detection(LOD)for this test method at asampling rate of 2 L/min are 0.042 g/m3for 1-h sampleduration and 0.005 g/m3for 8-h sample duration.1.6 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.7 This standard does not purport to address all the safetyconcerns,if any,associated with its use.It is the responsibilityof the user of this standard to establish appropriate safety andhealth practices and determine the applicability of regulatorylimitations prior to use.Specific precautionary information isgiven in 13.6.2.Referenced Documents2.1 ASTM Standards:2D1356 Terminology Relating to Sampling and Analysis ofAtmospheresD1357 Practice for Planning the Sampling of the AmbientAtmosphereD3631 Test Methods for Measuring Surface AtmosphericPressureD5337 Practice for Flow Rate Adjustment of Personal Sam-pling PumpsD5955 Test Methods for Estimating Contribution of Envi-ronmental Tobacco Smoke to Respirable Suspended Par-ticles Based on UVPM and FPM3.Terminology3.1 DefinitionsFor definitions of terms used in this testmethod,refer to Terminology D1356.3.2 Definitions of Terms Specific to This Standard:3.2.1 environmental tobacco smoke(ETS)an aged,dilutecomposite of exhaled tobacco smoke(exhaled mainstreamsmoke)and smoke from tobacco products(sidestream smoke).3.2.2 respirable suspended particles(RSP)particles whichcan be deposited in the gas-exchange region of the lung and aredefined as particles that pass through a sampler having a4.0-m median cutpoint(1).33.2.3 solanesol particulate matter(Sol-PM)a tobacco-selective marker for the contribution of ETS particulate matterto RSP.4.Summary of Test Method4.1 A known volume of air is drawn through an inertialimpactor or cyclone assembly separating at 4.0 m to separate1This test method is under the jurisdiction of ASTM Committee D22 on AirQuality and is the direct responsibility of Subcommittee D22.05 on Indoor Air.Current edition approved March 1,2016.Published March 2016.Originallyapproved in 1998.Last previous edition approved in 2010 as D6271 10.DOI:10.1520/D6271-10R16.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3The boldface numbers in parentheses refer to a list of references at the end ofthis standard.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 respirable suspended particles(RSP)from total suspendedparticulate matter and then through a filter assembly.Solanesolis collected as a component of RSP on a PTFE membrane filtercontained within the filter assembly.4.2 Solanesol is extracted from the filter with methanol in a4-mL glass vial.4.3 An aliquot of the extract is injected into an HPLCsystem equipped with a UV detector(205 nm absorbance).4.4 The area of the resulting solanesol peak is compared toareas obtained from the injection of standard solutions ofsolanesol,and the weight of solanesol is determined.4.5 The concentration of solanesol(g/m3)is calculatedfrom the weight of solanesol and t