ASTM_D_6530_-_19.pdf

Designation:D653019Standard Test Method forTotal Active Biomass in Cooling Tower Waters(Kool KountAssay;KKA)1This standard is issued under the fixed designation D6530;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope*1.1 This test method covers the determination of viableactive biomass in cooling tower water in the range from 102to108cfu/mL.It is a semiquantitative test method.1.2 This test method was used successfully with reagentwater,physiologic saline,and cooling tower waters.It is theusers responsibility to ensure the validity of this test methodfor waters of untested matrices.1.3 The values stated in SI units are to be regarded asstandard.The values given in parentheses after SI units areprovided for information only and are not considered standard.1.4 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.For specific hazard statements,see Section 9.1.5 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:2D1129 Terminology Relating to WaterD1192 Guide for Equipment for Sampling Water and Steamin Closed Conduits(Withdrawn 2003)3D1193 Specification for Reagent WaterD2777 Practice for Determination of Precision and Bias ofApplicable Test Methods of Committee D19 on WaterD3370 Practices for Sampling Water from Flowing ProcessStreams3.Terminology3.1 Definitions:3.1.1 For definitions of terms used in this standard,refer toTerminology D1129.3.2 Definitions of Terms Specific to This Standard:3.2.1 cfu/mL,ncolony forming units per millilitre.3.2.2 snapping cup,ncontainer provided for holding thesample and snapping tip of the vial.3.2.3 vial,nsealed glass ampoule under vacuum contain-ing reagents for the Kool Kount Test.4.Summary of Test Method4.1 This test method consists of adding a specific volume ofwater to nutrients and a color indicator contained in a glassvial.The contents of the vial are then mixed and incubated at35 6 3C(95F);that is,in a shirt pocket,incubator,or heatblock.The color of the sample after addition into the vialcontaining the nutrients and color indicator is yellow.Viableactive biomass in the sample replicates using the nutrientsprovided and reduces the color indicator.At a critical biomassconcentration,sufficient quantities of the color indicator arereduced resulting in a visible change in the indicator from theoriginal yellow sample color to orange.The time required forconversion of the oxidized indicator to the reduced indicatorresulting in an orange color as directly correlated with theconcentration of viable active biomass in the water sampletested.High concentrations of active biomass in the sampleproduce the positive orange color more rapidly than lowconcentrations of viable biomass.5.Significance and Use5.1 This test method is useful for rapid determination ofviable active biomass concentrations in cooling tower waters.The efficiency of cooling towers is directly affected by theconcentration of biomass in the cooling tower waters.Asbiomass concentrations increase,biofilm formation occurs1This test method is under the jurisdiction of ASTM Committee D19 on Waterand is the direct responsibility of Subcommittee D19.24 on Water Microbiology.Current edition approved Dec.1,2019.Published December 2019.Originallyapproved in 2000.Last previous edition approved in 2013 as D6530 00(2013).DOI:10.1520/D6530-19.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3The last approved version of this historical standard is referenced onwww.astm.org.*A Summary of Changes section appears at the end of this standardCopyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.resulting in a decrease in the efficiency of heat exchange in thetower.Current tests for monitoring the biomass conce