Designation:E2657−11AnAmericanNationalStandardStandardTestMethodforDeterminationofEndotoxinConcentrationsinWater-MiscibleMetalworkingFluids1ThisstandardisissuedunderthefixeddesignationE2657;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethodcoversquantitativemethodsforthesamplinganddeterminationofbacterialendotoxinconcentra-tionsinwatermisciblemetalworkingfluids(MWF).1.2UsersofthistestmethodneedtobefamiliarwiththehandlingofMWF.1.3Thismethodgivesanestimateoftheendotoxinconcen-trationinthesampledMWF.1.4ThismethodreplacesTestMethodE2250.1.5Thistestmethodseekstominimizeinter-laboratoryvariationofendotoxindatabutdoesnotensureuniformityofresults.1.6Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:2D4840GuideforSampleChain-of-CustodyProceduresE1497PracticeforSelectionandSafeUseofWater-MiscibleandStraightOilMetalRemovalFluidsE1542TerminologyRelatingtoOccupationalHealthandSafety2.2GovernmentStandard:329CFR1910.1450OccupationalExposuretoHazardousChemicalsinLaboratories2.3OtherDocuments:4CriteriaDocumentforaRecommendedStandard:Occupa-tionalExposuretoMetalworkingFluids,1998NIOSHManualofAnalyticalMethods(NMAM),4thed.,EllerandCassinelli,Eds.,19943.Terminology3.1DefinitionsofTermsSpecifictoThisStandard:3.1.1controlstandardendotoxin(CSE),n—apurifiedpreparationofendotoxinbasedontheUSPReferenceStandardEndotoxin(RSE);usedinlaboratoriestopreparestandardsolutions.3.1.2endotoxin,n—pyrogenichighmolarmasslipopolysac-charide(LPS)complexassociatedwiththecellwallofgram-negativebacteria.3.1.2.1Discussion—Thoughendotoxinsarepyrogens,notal...
