ASTM_D_7574_-_09.pdf

Designation:D757409Standard Test Method forDetermination of Bisphenol A in Environmental Waters byLiquid Chromatography/Tandem Mass Spectrometry1This standard is issued under the fixed designation D7574;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This procedure covers the determination of bisphenol A(BPA)extracted from water utilizing solid phase extraction(SPE),separated using liquid chromatography(LC)and de-tected with tandem mass spectrometry(MS/MS).BPA isqualitatively and quantitatively determined by this method.This method adheres to multiple reaction monitoring(MRM)mass spectrometry.1.2 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.3 The method detection limit(MDL)and reporting limit(RL)for BPA are listed in Table 1.1.4 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D1193 Specification for Reagent WaterD3694 Practices for Preparation of Sample Containers andfor Preservation of Organic ConstituentsD3856 Guide for Management Systems in LaboratoriesEngaged in Analysis of WaterD5847 Practice for Writing Quality Control Specificationsfor Standard Test Methods for Water AnalysisD5905 Practice for the Preparation of Substitute Wastewater3.Terminology3.1 Definitions:3.1.1 environmental water,neffluents from secondary andtertiary publicly owned treatment works(POTW),fresh sur-face and ground water.3.2 Abbreviations:3.2.1 pptparts per trillion,ng/L4.Summary of Test Method4.1 This is a performance based method and modificationsare allowed to improve performance.4.2 Solid phase extraction is used to extract water samples.4.2.1 Solid Phase Extraction250 milliliter volume ofsample adjusted to pH 2 is extracted using a solid phaseextraction cartridge.The resulting methyl tert-butyl ether(MTBE)extract is solvent exchanged into methanol,concen-trated to a volume of 0.2 mL,brought to a final volume of 1 mLwith water and then analyzed by LC/MS/MS operated in themultiple reaction monitoring(MRM)mode.4.3 The target compound,surrogate and internal standardsare identified by retention time and two SRM transitions.Thetarget analyte and surrogate are quantitated using the primarySRM transitions utilizing internal standard calibration.Thefinal report issued for each sample lists the concentration ofBPA and the bisphenol A(Ring-13C12)surrogate recovery.5.Significance and Use5.1 The first reported synthesis of BPA was by the reactionof phenol with acetone by Zincke.3BPA has become animportant high volume industrial chemical used in the manu-facture of polycarbonate plastic and epoxy resins.Polycarbon-ate plastic and resins are used in numerous products includingelectrical and electronic equipment,automobiles,sports andsafety equipment,reusable food and drink containers,electricallaminates for printed circuit boards,composites,paints,adhesives,dental sealants,protective coatings and many otherproducts.45.2 The environmental source of BPA is predominantlyfrom the decomposition of polycarbonate plastics and resins.1This test method is under the jurisdiction of ASTM Committee D19 on Waterand is the direct responsibility of Subcommittee D19.06 on Methods forAnalysis forOrganic Substances in Water.Current edition approved Oct.1,2009.Published October 2009.DOI:10.1520/D7574-09.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3Zincke,T.,1905,“Mittheilungen aus dem chemischen Laboratorium derUniversitat Marburg,”Justus Leibigs Annals Chemie,Vol.343,pages 7579.4Additional information about BPA is available at http:/www.bisphenol-a.org(2008).Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 BPA is not classified as bio-accumulative by the U.S.Environ-mental Protection Agency and will biodegrade.BPA has beenreported to have adverse effects in aquatic organisms and maybe released into environmental waters directly at trace levelsthrough landfill leachate and POTW effluents.This method hasbeen investigated for use with surface water and secondary andtertiary POTW effluent samples therefore,it is applicable tothese matrices only.It has not been investigated for use withsalt water or solid sample matrices.6.Interferences6.1 Method interferences may be caused by contaminants insolvents,reagents,glassware and