ASTM_D_6081_-_98_2014.pdf

Designation:D608198(Reapproved 2014)Standard Practice forAquatic Toxicity Testing of Lubricants:Sample Preparationand Results Interpretation1This standard is issued under the fixed designation D6081;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This practice covers procedures to be used in thepreparation of lubricants or their components for toxicitytesting in aquatic systems and in the interpretation of the resultsof such tests.1.2 This practice is suitable for use on fully-formulatedlubricants or their components that are not completely solubleat the intended test treat rates.It is also suitable for use withadditives,if the additive is tested after being blended into acarrier fluid at the approximate concentration as in the intendedfully formulated lubricant.The carrier fluid shall meet theabove solubility criterion,be known to be minimally toxic inthe toxicity test in which the material will be tested,and beknown to have a chemical composition similar to the rest of theintended fully formulated lubricant.1.3 Samples prepared in accordance with this practice maybe used in acute or chronic aquatic toxicity tests conducted infresh water or salt water with fish,large invertebrates,or algae.This practice does not address preparation of samples for planttoxicity testing other than algae.1.4 Standard acute and chronic aquatic toxicity proceduresare more appropriate for lubricants with compositions that arecompletely soluble at the intended test treat rates(1,2,3,4,5).21.5 This practice is intended for use with lubricants orlubricant components of any volatility.1.6 This practice does not address any questions regardingthe effects of any lubricant or lubricant component on humanhealth.1.7 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.8 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:3D1129 Terminology Relating to WaterD1193 Specification for Reagent WaterE729 Guide for Conducting Acute Toxicity Tests on TestMaterials with Fishes,Macroinvertebrates,and Amphib-iansE943 Terminology Relating to Biological Effects and Envi-ronmental Fate3.Terminology3.1 Definitions:3.1.1 acute toxicity test,na comparative toxicity test inwhich a representative subpopulation of organisms is exposedto different treat rates of a test material and is observed for ashort period,usually not constituting a substantial portion oftheir life span.3.1.2 chronic toxicity test,na comparative toxicity test inwhich a representative subpopulation of organisms is exposedto different treat rates of a test material and is observed for aperiod of time that constitutes a major portion of their life span.3.1.3 ecotoxicity,npropensity of a test material to produceadverse behavioral,biochemical,or physiological effects innon-human organisms or populations.3.1.4 effect load XX(ELXX),na statistically or graphicallyestimated loading rate of test material that is expected to causeone or more specified effects in XX%of a representativesubpopulation of organisms under specified conditions.3.1.4.1 DiscussionThis terminology should be used in-stead of the standard ECXX when the test material is notcompletely soluble at the test treat rates.1This practice is under the jurisdiction of ASTM Committee D02 on PetroleumProducts,Liquid Fuels,and Lubricants and is the direct responsibility of Subcom-mittee D02.12 on Environmental Standards for Lubricants.Current edition approved Oct.1,2014.Published November 2014.Originallyapproved in 1998.Last previous edition approved in 2009 as D608198(2009).DOI:10.1520/D6081-98R14.2The boldface numbers in parentheses refer to the list of references at the end ofthis standard.3For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 3.1.5 inhibition load XX(ILXX),na statistically or graphi-cally estimated loading rate of test material that is expected tocause a XX%inhibition of a biological process(such asgrowth or reproduction)that has an analog as opposed to adigital measure.3.1.5.1 DiscussionAn example of a digital measure wouldbe alive/dead.This terminology(ILXX)should be used insteadof the standard ICXX when the test material is not completelysoluble at the t