ASTM_D_8243_-_19.pdf

Designation:D824319Standard Test Method forDetermination of APS Reductase to Estimate SulfateReducing Bacterial Bioburdens in Water Enzyme-LinkedImmunosorbent Assay Method1This standard is issued under the fixed designation D8243;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method provides a protocol for using enzyme-linked immunosorbent assay(ELISA)technology to test watersamples for the enzyme adenosine 5-phosphosulfate reductase(APSr)concentration.1.1.1 APSr is present in all known sulfate reducing protists(SRP sulfate reducing bacteria SRB and sulfate reducingarchaea SRA).1.1.2 As reported in U.S.Patent 4,999,286,APS reductaseconcentration can be used as a surrogate parameter for esti-mating SRA bioburdens(Appendix X1 compares results fromTest Methods D8243,D4412,and quantitative polymerasechain reaction qPCR testing).1.2 This test method has been validated in tap water,oilfieldproduced water(salinities ranging from 100 g L-1to 600 g L-1),and fuel-associated water(commonly referred to as bottoms-water).1.3 This test method detects APS reductase semi-quantitatively in the range of 0.001M to 0.1M correlating to102SRP/mL to 106SRP/mL.1.3.1 As described in Appendix X2 test method sensitivitycan be increased 10-fold to 100-fold.However,the precisionstatistics provided in X apply only to 10-mL specimens.1.4 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.5 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.Some specific hazards statements are given in Section 9 onHazards.1.6 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:2D1129 Terminology Relating to WaterD4412 Test Methods for Sulfate-Reducing Bacteria in Waterand Water-Formed DepositsD5847 Practice for Writing Quality Control Specificationsfor Standard Test Methods for Water AnalysisD6300 Practice for Determination of Precision and BiasData for Use in Test Methods for Petroleum Products andLubricantsD6499 Test Method for Immunological Measurement ofAntigenic Protein in Hevea Natural Rubber(HNR)and itsProductsD7464 Practice for Manual Sampling of Liquid Fuels,As-sociated Materials and Fuel System Components forMicrobiological TestingE691 Practice for Conducting an Interlaboratory Study toDetermine the Precision of a Test MethodE1601 Practice for Conducting an Interlaboratory Study toEvaluate the Performance of an Analytical MethodE1847 Practice for Statistical Analysis of Toxicity TestsConducted Under ASTM GuidelinesE1914 Practice for Use of Terms Relating to the Develop-ment and Evaluation of Methods for Chemical Analysis(Withdrawn 2016)32.2 Patents:4U.S.Patent 4,999,286 Sulfate Reducing Bacteria Determi-nation and Control,March 12,19911This test method is under the jurisdiction of ASTM Committee D19 on Waterand is the direct responsibility of Subcommittee D19.24 on Water Microbiology.Current edition approved Jan.1,2019.Published February 2019.DOI:10.1520/D8243-19.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3The last approved version of this historical standard is referenced onwww.astm.org.4Available from United States Patent and Trademark Office(USPTO),MadisonBuilding,600 Dulany Street,Alexandria,VA 22314,https:/www.uspto.gov.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.1 3.Terminology3.1 Definitions:3.1.1 For definitions of terms used in this standard,refer toTerminology D1129.3.1.2 adenosine5-phosphosulfatereductase(APSreductase),n(EC 1.8.4.9 and CAS 9027-75-2)an enzymepresent on all known sulfate reducing bacteria and archaea,catalyzing the two-electron reduction of the sulfate group inadenosine 5-phosphosulfate(APS)to y