ASTM_D_8068_-_19.pdf

Designation:D806819Standard Practice forCollection of Culturable Airborne Fungi or Bacteria on AgarPlates by Inertial Impaction Systems1This standard is issued under the fixed designation D8068;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.INTRODUCTIONThis practice was introduced to bring uniformity to the process of sample collection for culturablefungi or bacteria using inertial impaction samplers for analysis by culture and optical microscopy.1.Scope1.1 The purpose of this practice is to describe procedures forthe collection of culturable airborne fungal spores or fragmentsor bacteria on agar plates using inertial impaction samplingtechniques.1.2 This practice does not include collection of culturablefungi or bacteria by devices not using agar plates.1.3 This practice presumes that the user has a fundamentalunderstanding of field investigative techniques related to thescientific process,and sampling plan development and imple-mentation.It is important to establish the related hypothesis tobe tested and the supporting analytical methodology needed inorder to identify the sampling media to be used and thelaboratory conditions for analysis.1.4 This practice does not address the development of aformal hypothesis or the establishment of appropriate anddefensible investigation and sampling objectives.It is pre-sumed the investigator has the experience and knowledge baseto address these issues.1.5 This practice does not provide the user sufficient infor-mation to allow for interpretation of the analytical results fromsample collection.It is the users responsibility to seek orobtain the information and knowledge necessary to interpretthe sample results reported by the laboratory.1.6 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.7 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.1.8 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:2D1356 Terminology Relating to Sampling and Analysis ofAtmospheresD3195/D3195M Practice for Rotameter CalibrationD6044 Guide for Representative Sampling for Managementof Waste and Contaminated MediaF1671 Test Method for Resistance of Materials Used inProtective Clothing to Penetration by Blood-Borne Patho-gens Using Phi-X174 Bacteriophage Penetration as a TestSystem3.Terminology3.1 DefinitionsFor definitions of terms used in thispractice,see Terminology D1356.3.1.1 agar,na semisolid culture medium used to supportthe growth of bacteria and other micro-organisms.F16713.1.2 sample,na portion of a population.3.1.2.1 DiscussionA portion of material that is taken fortesting or record purposes.D60443.1.3 sample,representative,na sample collected in sucha manner that it reflects the characteristics of interest of a1This practice is under the jurisdiction ofASTM Committee D22 on Air Qualityand is the direct responsibility of Subcommittee D22.08 on Assessment,Sampling,and Analysis of Microorganisms.Current edition approved March 1,2019.Published March 2019.Originallyapproved in 2018.Last previous edition approved in 2018 as D8068 18.DOI:10.1520/D8068-19.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.1 population(as defined)from which it is collected.adapted from D60443.1.3.1 DiscussionA population in the context of thisstandard refers to culturable fungi-or bacteria colony-formingunits that exist at the time and location of sampling.3.1.4 sampling train,nthe assemblage of equipment nec-essary to sample atmospheres.D13563.1.4.1 DiscussionComplete assembly from the pumpsystem through to the agar plate including any transport tubingand connectors