ASTM_D_7391_-_09.pdf

Designation:D739109Standard Test Method forCategorization and Quantification of Airborne FungalStructures in an Inertial Impaction Sample by OpticalMicroscopy1This standard is issued under the fixed designation D7391;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method is a procedure that uses direct micros-copy to analyze the deposit on an inertial impaction sample.1.2 This test method describes procedures for categorizingand enumerating fungal structures by morphological type.Typically,categories may be as small as genus(for example,Cladosporium)or as large as phylum(for example,basidi-ospores).1.3 This method contains two procedures for enumeratingfungal structures:one for slit impaction samples and one forcircular impaction samples.This test method is applicable forimpaction air samples,for which a known volume of air(at arate as recommended by the manufacturer)has been drawn,and is also applicable for blank impaction samples.1.4 Enumeration results are presented in fungal structures/sample(fs/sample)and fungal structures/m3(fs/m3).1.5 The range of enumeration results that can be determinedwith this method depends on the size of the spores on thesample trace,the amount of particulate matter on the sampletrace,the percentage of the sample trace counted,and thevolume of air sampled.1.6 This method addresses only the analysis of samples.Thesampling process and interpretation of results is outside thescope of this method.1.7 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.8 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D1193 Specification for Reagent Water3.Terminology3.1 ASTM Definitions(see ASTM Terminology Dictionary):3.1.1 numerical aperture3.2 Definitions of Terms Specific to This Standard:3.2.1 circular impaction sample,na sample of airborneparticulate matter collected via a device that draws air througha round aperture at a specified rate,impacting the particlessuspended in the air onto an adhesive medium,resulting in acircular area of deposition.Acircular impaction sample may becollected by means of a cassette manufactured for that purpose,or by means of a sampling device that requires slides to bepre-coated with impaction medium.3.2.2 debris rating,na distinct value assigned to animpactor sample based on the percentage of the sample areapotentially obscured by particulate matter,and ranging from 0to 5.3.2.3 field blank,na sample slide or cassette carried to thesampling site,exposed to sampling conditions(for example,seals opened),returned to the laboratory,treated as a sample,and carried through all steps of the analysis.3.2.4 hyaline,adjcolorless.3.2.5 impaction medium,na substance applied to a micro-scope slide used to collect(or capture)particulate matterduring sampling.3.2.6 impaction sample,na sample taken using impaction,for example,slit impaction sample,circular impaction sample.3.2.7 inertial impactor,na device for collecting particlesseparated from an air stream by inertia to force an impact ontoan adhesive surface.Inertial impactors are available in many1This test method is under the jurisdiction of ASTM Committee D22 on AirQuality and is the direct responsibility of Subcommittee D22.08 on Sampling andAnalysis of Mold.Current edition approved May 1,2009.Published June 2009.DOI:10.1520/D7391-09.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 designs,including those having a slit jet,yielding a rectangularsample trace,and a circular jet,yielding a circular sampletrace.3.2.8 magnification/resolution combination 1,n150-400 total magnification and a point to point resolution of 0.7m or better,as checked by a resolution check slide.3.2.9 magnification/resolution combination 2,n400 orgreater total magnification and a point to point resolution of 0.5m or better,as checked by a resolution check slide.3.2.10 minimum reporting limit(fs/sample);minimum re-porting limit(fs/m3),nthe lowest result to be reported fortotal spores or any spore category.Since both fs/sample andfs/m3are reported,there are two minimum reporting limits.3.2.11 morphology,nthe form and structure of an organ-ism or any of its parts;for