ASTM_D_6731_-_22.pdf

Designation:D673122Standard Test Method forDetermining the Aerobic,Aquatic Biodegradability ofLubricants or Lubricant Components in a ClosedRespirometer1This standard is issued under the fixed designation D6731;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope*1.1 This test method covers a procedure for determining thedegree of biodegradability of lubricants or their components inan aerobic aqueous medium on exposure to an inoculum undercontrolled laboratory conditions.This test method is an ulti-mate biodegradation test that measures oxygen demand in aclosed respirometer.1.2 This test method is suitable for evaluating the biodeg-radation of volatile as well as nonvolatile lubricants or lubri-cant components.1.3 This test method is applicable to lubricants and lubricantcomponents which are not toxic and not inhibitory to the testmicroorganisms at the test concentration.1.4 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.5 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.Specific hazards are given in Section 10.1.6 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:2D1129 Terminology Relating to WaterD1193 Specification for Reagent WaterD1293 Test Methods for pH of WaterD4175 Terminology Relating to Petroleum Products,LiquidFuels,and LubricantsD4447 Guide for Disposal of Laboratory Chemicals andSamplesD6384 Terminology Relating to Biodegradability and Eco-toxicity of LubricantsE943 Terminology Relating to Biological Effects and Envi-ronmental Fate2.2 ISO Standards:3ISO 42591,2 Petroleum productsDetermination and appli-cation of precision data in relation to methods of testISO 6107-2 Water qualityVocabularyPart 2ISO 8192 Water qualityTest for inhibition of oxygen con-sumption by activated sludge for carbonaceous and am-monium oxidationISO 9408 Water qualityEvaluation of ultimate aerobic bio-degradability of organic compounds in aqueous mediumby determination of oxygen demand in a closed respirom-eter2.3 OECD Standards:4OECD 301F Ready Biodegradability-Manometric Re-spirometry1This test method is under the jurisdiction of ASTM Committee D02 onPetroleum Products,Liquid Fuels,and Lubricantsand is the direct responsibility ofSubcommittee D02.12 on Environmental Standards for Lubricants.Current edition approved April 1,2022.Published April 2022.Originallyapproved in 2001.Last previous edition approved in 2018 as D6731 18.DOI:10.1520/D6731-22.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3Available from American National Standards Institute(ANSI),25 W.43rd St.,4th Floor,New York,NY 10036,http:/www.ansi.org.4Available from Organisation for Economic Cooperation and Development(OECD),2 rue Andr Pascal,F-75775,Paris Cedex 16,France,http:/www.oecd.org.*A Summary of Changes section appears at the end of this standardCopyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.12.4 APHA Standards:52540B Total Solids Dried at 103-105C9215 Heterotrophic Plate Count3.Terminology3.1 Definitions:3.1.1 Definitions of terms applicable to this test methodappear in the Compilation of ASTM Standard Definitions andthe following terminology standards:D1129,D4175,D6384,E943,and ISO 6107-2.3.1.2 activated sludge,nthe precipitated solid matter,consistingmainlyofbacteriaandotheraquaticmicroorganisms,that is produced at a domestic wastewatertreatment plant and is used primarily in secondary sewagetreatment to microbially oxidize dissolved organic matter in theeffluent.3.1.3 aerobic,adj(a)taking place in the presence ofoxygen;(b)living or active in the presence of oxygen.3.1.4 biochemical oxygen demand(BOD),nthe massconcentration of dissolved ox