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Designation:D646920Standard Guide forMicrobial Contamination in Fuels and Fuel Systems1This standard is issued under the fixed designation D6469;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope*1.1 This guide provides personnel who have a limitedmicrobiological background with an understanding of thesymptoms,occurrence,and consequences of chronic microbialcontamination.The guide also suggests means for detectionand control of microbial contamination in fuels and fuelsystems.This guide applies primarily to gasoline,aviation,boiler,industrial gas turbine,diesel,marine,furnace fuels andblend stocks(see Specifications D396,D910,D975,D1655,D2069,D2880,D3699,D4814,D6227,and D6751),and fuelsystems.However,the principles discussed herein also applygenerally to crude oil and all liquid petroleum fuels.ASTMManual 472provides a more detailed treatment of the conceptsintroduced in this guide;it also provides a compilation of all ofthe standards referenced herein that are not found in the AnnualBook of ASTM Standards,Section Five on Petroleum Productsand Lubricants.1.2 This guide is not a compilation of all of the concepts andterminology used by microbiologists,but it does provide ageneral understanding of microbial fuel contamination.1.3 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.4 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:3D130 Test Method for Corrosiveness to Copper from Petro-leum Products by Copper Strip TestD396 Specification for Fuel OilsD445 Test Method for Kinematic Viscosity of Transparentand Opaque Liquids(and Calculation of Dynamic Viscos-ity)D515 Test Method for Phosphorus In Water(Withdrawn1997)4D664 Test Method for Acid Number of Petroleum Productsby Potentiometric TitrationD888 Test Methods for Dissolved Oxygen in WaterD910 Specification for Leaded Aviation GasolinesD974 Test Method for Acid and Base Number by Color-Indicator TitrationD975 Specification for Diesel FuelD1067 Test Methods for Acidity or Alkalinity of WaterD1126 Test Method for Hardness in WaterD1293 Test Methods for pH of WaterD1298 Test Method for Density,Relative Density,or APIGravity of Crude Petroleum and Liquid Petroleum Prod-ucts by Hydrometer MethodD1331 Test Methods for Surface and Interfacial Tension ofSolutions of Paints,Solvents,Solutions of Surface-ActiveAgents,and Related MaterialsD1426 Test Methods for Ammonia Nitrogen In WaterD1655 Specification for Aviation Turbine FuelsD1744 Test Method for Determination of Water in LiquidPetroleum Products by Karl Fischer Reagent(Withdrawn2016)4D1976 Test Method for Elements in Water by Inductively-Coupled Plasma Atomic Emission SpectroscopyD2068 Test Method for Determining Filter Blocking Ten-dencyD2069 Specification for Marine Fuels(Withdrawn 2003)4D2274 Test Method for Oxidation Stability of Distillate Fuel1This guide is under the jurisdiction of ASTM Committee D02 on PetroleumProducts,Liquid Fuels,and Lubricants and is the direct responsibility of Subcom-mittee D02.14 on Stability,Cleanliness and Compatibility of Liquid Fuels.Current edition approved Sept.1,2020.Published October 2020.Originallyapproved in 1999.Last previous edition approved in 2017 as D6469 17.DOI:10.1520/D6469-20.2MNL 47,Fuel and Fuel System Microbiology:Fundamentals,Diagnosis,andContamination Control,Passman,F.J.,ed.,ASTM International,2003.3For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.4The last approved version of this historical standard is referenced onwww.astm.org.*A Summary of Changes section appears at the end of this standardCopyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Tec