ASTM_D_7788_-_14.pdf

Designation:D778814Standard Practice forCollection of Total Airborne Fungal Structures via InertialImpaction Methodology1This standard is issued under the fixed designation D7788;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 The purpose of this practice is to describe procedures forthe collection of airborne fungal spores or fragments,or both,using inertial impaction sampling techniques.1.2 This practice is not intended to limit the user from thecollection of other airborne particulates that may be of interestand captured through this technique.1.3 This practice presumes that the user has a fundamentalunderstanding of field investigative techniques related to thescientific process,and sampling plan development and imple-mentation.It is important to establish the related hypothesis tobe tested and the supporting analytical methodology needed inorder to identify the sampling media to be used and thelaboratory conditions for analysis.1.4 This practice does not address the development of aformal hypothesis or the establishment of appropriate anddefensible investigation and sampling objectives.It is pre-sumed the investigator has the experience and knowledge baseto address these issues.1.5 This practice does not provide the user sufficient infor-mation to allow for interpretation of the analytical results fromsample collection.It is the users responsibility to seek orobtain the information and knowledge necessary to interpretthe sample results reported by the laboratory.1.6 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.7 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D1356 Terminology Relating to Sampling and Analysis ofAtmospheresD3195/D3195M Practice for Rotameter CalibrationD4840 Guide for Sample Chain-of-Custody ProceduresD6044 Guide for Representative Sampling for Managementof Waste and Contaminated MediaD7391 Test Method for Categorization and Quantification ofAirborne Fungal Structures in an Inertial ImpactionSample by Optical Microscopy3.Terminology3.1 DefinitionsFor definitions and terms not listed here,see Terminology D1356.3.1.1 inertial impactor,na device designed for the impac-tion of particles that are separated from the air stream by inertiaonto a collection surface.D73913.1.1.1 DiscussionInertial impactors are available inmany designs including“slit”and“circular”jets.3.1.1.2 DiscussionAllows for the identification to genusor group of fungi detected,quantification to spores/m3,andgeneral assessment of background debris.Identification ofpollen,hyphal fragments and other airborne particulate may beincluded.3.1.2 sample,na portion of a population.A portion ofmaterial that is taken for testing or record purposes.D60443.1.3 sample,representative,na sample collected in sucha manner that it reflects one or more characteristics of interest(as defined by the project objectives)of a population fromwhich it is collected.D60443.1.3.1 DiscussionPopulations of airborne fungal sporesare typically not homogeneous.3.2 Definitions of Terms Specific to This Standard:3.2.1 aerodynamic diameter(da),nthe diameter of a unitdensity sphere having the same inertial properties as theparticle under analysis under the same conditions.1This practice is under the jurisdiction ofASTM Committee D22 on Air Qualityand is the direct responsibility of Subcommittee D22.08 on Sampling and Analysisof Mold.Current edition approved June 1,2014.Published July 2014.DOI:10.1520/D7788-14.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 3.2.1.1 DiscussionFor fungal spores this is generallybased on a water droplet,(spherical particle)having a densityof 1 g/cm3.Aerodynamic diameter has been developed tocategorize the sizes of particles of different shapes and densi-ties with a single dimension.The aerodynamic diameter is notnecessarily equal to the physical diameter due to variations inshape or density.3.2.2 calibration impactor,na designated cassette,ormedia unit,placed in the sampling assembly during calibrationor verification of the air flow rate.3.2.3 chain of custody(COC)record,na document thatprovides for the traceable transfer of field samples to theanaly