ASTM_D_7391_-_20.pdf

Designation:D739120Standard Test Method forCategorization and Quantification of Airborne FungalStructures in an Inertial Impaction Sample by OpticalMicroscopy1This standard is issued under the fixed designation D7391;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method is a procedure that uses direct micros-copy to analyze the deposit on an inertial impaction sample.1.2 This test method describes procedures for categorizingand enumerating fungal structures by morphological type.Typically,categories may be as small as genus(for example,Cladosporium)or as large as phylum(for example,basidi-ospores).1.3 This test method contains two procedures for enumer-ating fungal structures:one for slit impaction samples and onefor circular impaction samples.This test method is applicablefor impaction air samples,for which a known volume of air(ata rate as recommended by the manufacturer)has been drawn,and is also applicable for blank impaction samples.1.4 Enumeration results are presented in fungal structures/sample(fs/sample)and fungal structures/m3(fs/m3).1.5 The range of enumeration results that can be determinedwith this test method depends on the size of the spores on thesample trace,the amount of particulate matter on the sampletrace,the percentage of the sample trace counted,and thevolume of air sampled.1.6 This test method addresses only the analysis of samples.The sampling process and interpretation of results is outsidethe scope of this test method.1.7 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.8 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.1.9 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:2D1193 Specification for Reagent WaterE691 Practice for Conducting an Interlaboratory Study toDetermine the Precision of a Test Method3.Terminology3.1 ASTM Definitions(see the ASTM Online Dictionary ofEngineering Science and Technology3):3.1.1 numerical aperture.3.2 Definitions of Terms Specific to This Standard:3.2.1 circular impaction sample,na sample of airborneparticulate matter collected by means of a device that draws airthrough a round aperture at a specified rate,impacting theparticles suspended in the air onto an adhesive medium,resulting in a circular area of deposition.A circular impactionsample may be collected by means of a cassette manufacturedfor that purpose,or by means of a sampling device that requiresslides to be pre-coated with impaction medium.3.2.2 debris rating,na distinct value assigned to animpactor sample based on the percentage of the sample areapotentially obscured by particulate matter,and ranging from 0to 5.3.2.3 field blank,na sample slide or cassette carried to thesampling site,exposed to sampling conditions(for example,1This test method is under the jurisdiction of ASTM Committee D22 on AirQuality and is the direct responsibility of Subcommittee D22.08 on Assessment,Sampling,and Analysis of Microorganisms.Current edition approved March 15,2020.Published April 2020.Originallyapproved in 2009.Last previous edition approved in 2017 as D7391 171.DOI:10.1520/D7391-20.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3ASTM Online Dictionary of Engineering Science and Technology(Stock#:DEFONLINE)is available on the ASTM website,www.astm.org,or contact ASTMCustomer Service at serviceastm.org.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.seals opened),returned to the laboratory,treated as a sample,and carried through all steps of the analysis.3.2.4fungal structure(sing.),na collective term forfragments or groups of fragments from fungi,including but notlimited to coni