ASTM_D_6406_-_99_2014.pdf

Designation:D640699(Reapproved 2014)Standard Test Method forAnalysis of Sugar in Vegetable Tanning Materials1This standard is issued under the fixed designation D6406;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method covers determining the sugars presentin vegetable tanning materials.1.2 The values stated in SI units are to be regarded as thestandard.The inch-pound units given in parentheses are forinformation only.1.3 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D4901 Practice for Preparation of Solution of Liquid Veg-etable Tannin ExtractsD4905 Practice for Preparation of Solution of Solid,Pastyand Powdered Vegetable Tannin ExtractsD6401 Test Method for Determining Non-Tannins and Tan-nin in Extracts of Vegetable Tanning MaterialsD6403 Test Method for Determining Moisture in Raw andSpent MaterialsD6404 Practice for Sampling Vegetable Materials Contain-ing TanninD6405 Practice for Extraction of Tannins from Raw andSpent MaterialsD6408 Test Method for Analysis of Tannery Liquors2.2 ALCA Methods:A30 Sugar in Tanning Materials33.Terminology3.1 Definitions:3.1.1 dextrosed-glucose.3.1.2 glucosea simple sugar with formula C6H12O6,andknown to exist in d-,l-,and racemic forms.The termcommonly refers to the sweet,colorless,water-soluble dextro-rotatory form that occurs widely in nature and is the usual formin which carbohydrate is assimilated by animals.The termglucose can also refer to a light-colored syrup made from cornstarch.3.1.3 sugarany of various water-soluble compounds thatvary widely in sweetness and comprise the oligosaccharidesincluding sucrose.4.Summary of Test Method4.1 An analytical strength solution(that is,4.00 6 0.25 gtannin per litre)of the tanning material is analyzed for reducingsugars and total sugars by the Munson and Walker procedure.5.Significance and Use5.1 This test method is used to determine the quantity ofsugar present in vegetable tanning materials or vegetabletannin extracts.The amount of the reducing sugars,totalsugars,and non-reducing sugars in a sample of material orextract can be determined by this method.5.2 Because of the possibility of errors in this test method itis essential that the method be followed exactly in order toobtain reproducible results both among specimens within alaboratory and for analyses between laboratories.6.Apparatus and Reagents6.1 Saturated Solution of Normal Lead Acetate.6.2 DipotassiumHydrogenPhosphate,Anhydrous(K2HPO4),dried in an oven at 100C for 16 h then stored in atightly stoppered bottle.6.3 Toluene,assay 99.5%.6.4 Fehlings Solutions,A and B.6.5 Hydrochloric Acid,concentrated(sp.gr.1.18).6.6 Kerosene,commercial grade.6.7 Saturated Solution of Sodium Hydroxide.1This test method is under the jurisdiction of ASTM Committee D31 on Leatherand is the direct responsibility of Subcommittee D31.01 on Vegetable Leather.Thismethod has been adapted from and is a replacement for Method A30 of the OfficialMethods of the American Leather Chemists Association.Current edition approved Nov.1,2014.Published December 2014.Originallyapproved in 1999.Last previous edition approved in 2009 as D6406 99(2009).DOI:10.1520/D6406-99R14.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3Official Methods of the American Leather Chemists Association.Availablefrom the American Leather Chemists Association,University of Cincinnati,P.O.Box 210014,Cincinnati,OH 45221-0014.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 6.8 Phenolphthalein Solution,0.5 g dissolved in 100 mL of95%ethanol.6.9 Tartaric Acid,powdered.6.10 Copper Sulfate Solution,prepared by dissolving69.278 g of CuSO4 5H2O in 1 L of distilled water and filteringthrough asbestos.6.11 Alkaline Tartrate Solution,prepared by dissolving 346g of Rochelle salt(sodium potassium tartrate tetrahydrate)and100 g of sodium hydroxide in 1 L of distilled water.Afterstanding for two days the solution shall be filtered throughasbestos.6.12 Alcohol,95%ethyl alcohol.6.13 Ether,diethyl ether.6.14 Filter Paper4,21.5 cm diameter,pleated to contain 32evenly divided creases.6.15 Funnel,100-125 mm top diameter,60 angle bowl,and150 mm stem length.6.16 Watch Glasses,a suitable size(approximately 150 mmdiameter)to be used as a cover for the funnel and filter paper.6.17