ASTM_D_7687_-_21.pdf

Designation:D768721Standard Test Method forMeasurement of Cellular Adenosine Triphosphate in Fueland Fuel-associated Water With Sample Concentration byFiltration1This standard is issued under the fixed designation D7687;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope*1.1 This test method covers a protocol for capturing,ex-tracting and quantifying the cellular adenosine triphosphate(cellular-ATP)content associated with microorganisms foundin fuels and fuel-associated water.1.2 The ATP is measured using a bioluminescence enzymeassay,whereby light is generated in amounts proportional tothe concentration of cellular-ATP in the samples.The light isproduced and measured quantitatively as relative light units(RLU)which are converted by comparison with an ATPstandard,computation to pg ATP/mL and optional furthertransformation to Log10pg ATP/mL.1.3 This test method is equally suitable for use as alaboratory or portable method.1.4 This test method is limited to fuels with a nominalviscosity 75 cSt at test temperature.1.5 This test method detectsATPconcentrations in the rangeof 5.0 pg ATP/mL(0.699 log10pg ATP/mL)to 100 000 pgATP/mL(5.000 log10pgATP/mL)for 20 mLsamples of fueland 20 pg ATP/mL(1.301 log10pg ATP/mL)to 400 000 pgATP/mL(5.602 log10pg ATP/mL)for 5 mL samples offuel-associated water.NOTE1These ranges were calculated with the formula for calculatingsample ATP in pg/mL provided in 12.1 based on the minimum recom-mended RLU for a 1 ng mL ATP standard when using the reagentsspecified in Section 7 and the luminometer specified in 6.4 and correctedwith a reagent-method blank as determined in Appendix X5.1.6 Providing interferences can be overcome,biolumines-cence is a reliable and proven method for qualifying andquantifying ATP.This test method does not differentiatebetween ATP from different sources,for example:from differ-ent types of microorganisms,such as bacteria and fungi.1.7 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.8 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.1.9 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:2D396 Specification for Fuel OilsD975 Specification for Diesel FuelD1129 Terminology Relating to WaterD1655 Specification for Aviation Turbine FuelsD2069 Specification for Marine Fuels(Withdrawn 2003)3D2880 Specification for Gas Turbine Fuel OilsD3699 Specification for KerosineD4012 Test Method forAdenosine Triphosphate(ATP)Con-tent of Microorganisms in WaterD4175 Terminology Relating to Petroleum Products,LiquidFuels,and LubricantsD6161 Terminology Used for Microfiltration,Ultrafiltration,Nanofiltration,and Reverse Osmosis Membrane ProcessesD6300 Practice for Determination of Precision and BiasData for Use in Test Methods for Petroleum Products,Liquid Fuels,and LubricantsD6751 Specification for Biodiesel Fuel Blend Stock(B100)1This test method is under the jurisdiction of ASTM Committee D02 onPetroleum Products,Liquid Fuels,and Lubricants and is the direct responsibility ofSubcommittee D02.14 on Stability,Cleanliness and Compatibility of Liquid Fuels.Current edition approved April 1,2021.Published April 2021.Originallyapproved in 2011.Last previous edition approved in 2017 as D7687 17.DOI:10.1520/D7687-21.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3The last approved version of this historical standard is referenced onwww.astm.org.*A Summary of Changes section appears at the end of this standardCopyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.1for Middle Distillate FuelsD7463 Test Method forAdenosine Triphosphate(ATP)Con-tent of Microorganisms in Fuel,Fuel/Water Mixtures,and