ASTM_D_6592_-_01.pdf

Designation:D 6592 01An American National StandardStandard Test Method forPortable Chemiluminescent Water Quality Determination1This standard is issued under the fixed designation D 6592;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon(e)indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method covers the detection of water andwastewater contaminants which inhibit chemiluminescence.1.2 This test method may be applied to ambient watersincluding but not exclusively river water and water fromsewage treatment plants.It is the responsibility of the user ofthis standard to ensure the validity of the test method for watersof untested matrices.1.3 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:D 1129 Terminology Relating to Water2D 1192 Specification for Equipment for Sampling Waterand Steam in Closed Conduits2D 1193 Specification for Reagent Water2D 2777 Determination of Precision and Bias of ApplicableMethods of Committee D19 on Water2D 3370 Standard Practices for Committee D19 on SamplingWater2D 3856 Standard Guide for Good Laboratory Practices inLaboratories Engaged in Sampling and Analysis of Water2D 4210 Practice for Interlaboratory Quality Control Proce-dures and Discussion on Reporting Low Level Data2D 5847 Standard Practice for Writing Quality ControlSpecifications for Standard Test Methods for Water Analy-sis23.Terminology3.1 DefinitionsFor definitions of other terms used in thistest method,refer to D 1129 Terminology Relating To Water.3.2 Definitions of Terms Specific to This Standard:3.2.1 chemiluminescencethe generation of light by achemical reaction.3.2.2 luminometeran instrument capable of measuringlight emitted during a luminescent reaction,over a wide rangeof wavelengths.3.2.3 photodiodea semiconductor light sensor that gener-ates a current or voltage when illuminated by light.4.Summary of Test Method4.1 This analytical test method is an enhanced chemilumi-nescent method based on the procedure of Whitehead et al.3Luminol is oxidized by a complex formed between an oxidantand peroxidase(HRP)to form a luminol radical.This decom-poses via an endoperoxide intermediate to form the excitedstate 3-aminophthalate dianion which decays to its ground statewith the emission of light.The presence of enhancers results ina steady output of luminol radicals during the reaction(see Fig.1).Contaminating substances(such as sewage,silage effluent,heavy metals,detergents,cyanides and pesticides containingorganic residues)react with the assay reagents by scavengingradicals from the luminol or enhancer radicals,or by inhibitingthe catalytic action of HRP.This results in a reduction of lightoutput to a degree which is proportional to the amount ofcontaminant present.Light output is measured using a portableluminometer,which detects light by means of a silicon photo-diode.5.Significance and Use5.1 This test method was developed for the purpose ofscreening for contamination in water.It is used for the analysisof waste water,process water and environmental water.Resultsof testing performed in the pulp and paper industry,sewagetreatment plants and river water systems are presented inAppendix X1.5.2 This method is not suitable for determining the preciseconcentration of specific analytes.1This test method is under the jurisdiction of ASTM Committee D19 on Waterand is the direct responsibility of Subcommittee D19.06 on Methods forAnalysis forOrganic Substances in Water.Current edition approved March 10,2001.Published May 2001.2Annual Book of ASTM Standards,Vol 11.01.3Whitehead T.P.Thorpe G.,Lane M.,WatsonA.,Billings C.in Bioluminescenceand Chemiluminescence:status report,Szalay A.Z,Kricka L.N.and Stanley PE.Wiley 1993,pp.425-429.FIG.1 Output of Luminol Radicals1Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959,United States.5.3 This test method may be used to develop trend data,which can be useful in assessing the performance characteris-tics of treatment process or changes in the raw water.6.Interferences6.1 Particulate MatterSamples containing a high degreeof particulate matter4can interfere with the test.Particulate orsuspended matter may be removed by centrifugation or mem-brane filtration if components of interest are not altered.7.Apparatus7.1 A hand-held portable luminometer with key pad andLCD display capable of measuring light output over a 4 minuteperiod,and integrating the result.See Fig.2.8.Reagents8.1 Purity of ReagentsReagent grade chemicals shall beused in all tests.Unless otherwise indicated,i