ASTM_D_6363_-_98_2013.pdf

Designation:D636398(Reapproved 2013)Standard Test Method forDetermination of Hydrogen Peroxide and Combined OrganicPeroxides in Atmospheric Water Samples by PeroxidaseEnzyme Fluorescence Method1This standard is issued under the fixed designation D6363;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 Thistestmethodcoversthedeterminationofhydroperoxides,which include hydrogen peroxide(H2O2)andcombined organic peroxides,in samples of atmospheric waterby the method of horseradish peroxidase derivatization andfluorescence analysis of the derived dimer.2,31.2 The range of applicable hydrogen peroxide concentra-tions was determined to be 0.6-176.0 106M fromindependent laboratory tests of the test method.1.3 The primary use of the test method is for hydrogenperoxide,but it may also be used to quantitate organichydroperoxides.Determinations of organic hydroperoxide con-centration levels up to 30 106M may be adequatelyobtained by calibration with hydrogen peroxide.2,3Whileorganic hydroperoxides have not been detected at significantconcentration levels in rain or cloud water,their presence maybe tested by operation of the test method with the addition ofcatalase for destruction of H2O23.1.4 Because of the instability of hydroperoxides in atmo-spheric water samples,proper sample collection,at-collectionderivatization,and stringent quality control are essential as-pects of the analytical process.1.5 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:4D1129 Terminology Relating to WaterD1193 Specification for Reagent WaterD1356 Terminology Relating to Sampling and Analysis ofAtmospheresD5012 Guide for Preparation of Materials Used for theCollection and Preservation of Atmospheric Wet Deposi-tionD5111 Guide for Choosing Locations and Sampling Meth-ods to Monitor Atmospheric Deposition at Non-UrbanLocationsE200 Practice for Preparation,Standardization,and Storageof Standard and Reagent Solutions for Chemical Analysis3.Terminology3.1 DefinitionsFor definitions of terms used in this testmethod,refer to Terminologies D1129 and D1356 and GuideD5111.3.2 Definitions of Terms Specific to This Standard:3.2.1 atmospheric water,nliquid or solid water suspendedin the atmosphere or deposited from the atmosphere.Forms ofatmospheric water include rain,snow,fog,cloud water,dew,and frost.3.2.2 derivatization,nformationofthep-hydroxyphenylacetic acidic dimer by combination ofp-hydroxyphenylacetic acid,horseradish peroxidase reagent,and hydroperoxide(s).Also the procedure of addition of thederivatizing reagent to samples.3.2.3 hydroperoxides,nhydrogen peroxide and organicperoxides dissolved in water.3.2.4 intrinsic hydroperoxides,nhydroperoxides con-tained in reagent water used for the method.3.2.5 post-derivatization,naddition of the derivatizingreagent to the sample after collection.1This guide is under the jurisdiction of ASTM Committee D22 on Air Qualityand is the direct responsibility of Subcommittee D22.03 on Ambient Atmospheresand Source Emissions.Current edition approved Oct.1,2013.Published October 2013.Originallyapproved in 1998.Last previous edition approved in 2003 as D6363-98(09)1.DOI:10.1520/D6363-98R13.2Lazrus,A.L.,Kok,G.L.,Gitlin,S.N.,and Lind,J.A.,“AutomatedFluorometric Method for Hydrogen Peroxide in Atmospheric Precipitation,”Anal.Chem.,57,1985,pp.917922.3Kok,G.L.,Thompson,K.,and Lazrus,A.L.,“Derivatization Technique for theDetermination of Peroxides in Precipitation,”Anal.Chem.,58,1986,pp.11921194.4For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 3.2.6 pre-derivatization,naddition of the derivatizing re-agent to the sample collection container prior to samplecollection.3.2.7 systems blank,na field blank of reagent water that issubjected to a similar or identical environment and derivatiza-tion time as a collected atmospheric water sample.3.2.8 systems standard,na H2O2calibration standardsolution subjected to a similar or identical environment andderivatization time as a collected atmospheric water sample.4.Summary of Test Method4.1 The peroxidase enzyme fluorescence method is based onthe reaction of hydroperoxides,horseradish peroxidase,andp-hydroxyphenylacetic(PHOPAA)acid,forming a fluorescentdimer of the latter.This d