Designation:D7817−12StandardTestMethodforEnumerationofYeastandMoldinRacewayBrine,Brine-CuredHidesandSkins1ThisstandardisissuedunderthefixeddesignationD7817;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethodcoverstheenumerationofyeastandmold.Thistestmethodisapplicabletoracewaybrine,brine-curedhidesandskins,andpre-chargeracewayliquor.1.2ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:2D6715PracticeforSamplingandPreparationofFreshorSalt-Preserved(Cured)HidesandSkinsforChemicalandPhysicalTestsE691PracticeforConductinganInterlaboratoryStudytoDeterminethePrecisionofaTestMethodE177PracticeforUseoftheTermsPrecisionandBiasinASTMTestMethods3.SummaryofTestMethod3.1Samplesofbrine-curedhidesandskins,racewaybrineorpre-chargeracewayliquorareseriallydilutedandplatedonagarcontaining7%NaClandanantibioticsolution.Theplatesareincubatedat20–25°Cfor5days.4.SignificanceandUse4.1Thistestmethodenumeratessalttolerantyeastandmold,andundertheconditionsofthistestmethodthoseareequatedashalophilicorganisms.Salttolerantyeastandmoldhavebeenknowntocausedamagetohidesandskinsinracewaybrine.5.Apparatus5.1Incubator,20–25°C.5.2Colonycounter—(notmandatory,buthighlyrecom-mended).5.3Sterilepipets.5.4Stomacher,formixinginitialdilution.(Ifstomacherisunavailable,hand-mix.)5.5Balance.5.6Sterilepetridishes.5.7Autoclave(sterilizer)—(Checktheeffectivenessofster-ilizationweekly.Forexample,placesporesuspensionsorstripsofBacillusstearothermophilus(commer...
