ASTM_D_6363_-_20.pdf

Designation:D636320Standard Test Method forDetermination of Hydrogen Peroxide and Combined OrganicPeroxides in Atmospheric Water Samples by PeroxidaseEnzyme Fluorescence Method1This standard is issued under the fixed designation D6363;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 Thistestmethodcoversthedeterminationofhydroperoxides,which include hydrogen peroxide(H2O2)andcombined organic peroxides,in samples of atmospheric waterby the method of horseradish peroxidase derivatization andfluorescence analysis of the derived dimer.2,31.2 The range of applicable hydrogen peroxide concentra-tions was determined to be 0.6176.0 106M from indepen-dent laboratory tests of the test method.1.3 The primary use of the test method is for hydrogenperoxide,but it may also be used to quantitate organichydroperoxides.Determinations of organic hydroperoxide con-centration levels up to 30 106M may be adequatelyobtained by calibration with hydrogen peroxide.2,3Whileorganic hydroperoxides have not been detected at significantconcentration levels in rain or cloud water,their presence maybe tested by operation of the test method with the addition ofcatalase for destruction of H2O2.31.4 Because of the instability of hydroperoxides in atmo-spheric water samples,proper sample collection,at-collectionderivatization,and stringent quality control are essential as-pects of the analytical process.1.5 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.6 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.1.7 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:4D1129 Terminology Relating to WaterD1193 Specification for Reagent WaterD1356 Terminology Relating to Sampling and Analysis ofAtmospheresD5012 Guide for Preparation of Materials Used for theCollection and Preservation of Atmospheric Wet Deposi-tionD5111 Guide for Choosing Locations and Sampling Meth-ods to Monitor Atmospheric Deposition at Non-UrbanLocationsE200 Practice for Preparation,Standardization,and Storageof Standard and Reagent Solutions for Chemical Analysis3.Terminology3.1 DefinitionsFor definitions of terms used in this testmethod,refer to Terminologies D1129 and D1356 and GuideD5111.3.2 Definitions of Terms Specific to This Standard:3.2.1 atmospheric water,nliquid or solid water suspendedin the atmosphere or deposited from the atmosphere.Forms ofatmospheric water include rain,snow,fog,cloud water,dew,and frost.3.2.2 derivatization,nformationofthep-hydroxyphenylacetic acidic dimer by combination ofp-hydroxyphenylacetic acid,horseradish peroxidase reagent,1This test method is under the jurisdiction of ASTM Committee D22 on AirQuality and is the direct responsibility of Subcommittee D22.03 on AmbientAtmospheres and Source Emissions.Current edition approved Sept.1,2020.Published September 2020.Originallyapproved in 1998.Last previous edition approved in 2013 as D6363 98(2013).DOI:10.1520/D6363-20.2Lazrus,A.L.,Kok,G.L.,Gitlin,S.N.,and Lind,J.A.,“AutomatedFluorometric Method for Hydrogen Peroxide in Atmospheric Precipitation,”Ana-lytical Chemistry,Vol 57,1985,pp.917922.3Kok,G.L.,Thompson,K.,and Lazrus,A.L.,“Derivatization Technique for theDetermination of Peroxides in Precipitation,”Analytical Chemistry,Vol 58,1986,pp.11921194.4For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.1and hydroperoxide(s).Also the procedure of addition of thederivatizing reagent to samples.3.2.3 hydroperoxides,nhydrogen peroxide and organicperoxides dissolved in water.3.2.4 intrinsic hydroperoxides,nhydroperoxides con-tained in reagent water used for t