Designation:D7304−22StandardTestMethodforDeterminationofDenatoniumBenzoateinEngineCoolantbyHPLC1ThisstandardisissuedunderthefixeddesignationD7304;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethodcoversthechemicalanalysisofenginecoolantfordenatoniumbenzoate(DNB)byhigh-performanceliquidchromatography(HPLC).DNBisaddedtopotentiallyrenderenginecoolantunpalatabletoanimalsandhumans.ThisanalyticalmethodwasdesignedfortheanalysisofDNBandisnotvalidforanyotherbitteringagentssuchasdenatoniumsaccharide.1.2Thistestmethodisapplicabletobothnewandusedcoolants.1.3Coelutionofotherionsmaycauseinterferencesinthedetectionofthedenatoniumcation.Inthecaseofunfamiliarformulations,identificationverificationshouldbeperformedbyeitherorbothfortificationanddilutionofthesamplematrixwithdenatoniumion.1.4ThevaluesstatedinSIunitsaretoberegardedasstandard.Thevaluesgiveninparenthesesareforinformationonly.1.5Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafety,health,andenvironmentalpracticesanddeter-minetheapplicabilityofregulatorylimitationspriortouse.1.6Thisinternationalstandardwasdevelopedinaccor-dancewithinternationallyrecognizedprinciplesonstandard-izationestablishedintheDecisiononPrinciplesfortheDevelopmentofInternationalStandards,GuidesandRecom-mendationsissuedbytheWorldTradeOrganizationTechnicalBarrierstoTrade(TBT)Committee.2.ReferencedDocuments2.1ASTMStandards:2D1193SpecificationforReagentWaterD1176PracticeforSamplingandPreparingAqueousSolu-tionsofEngineCoolantsorAntirustsforTestingPurposesE177PracticeforUseoftheTermsPrecisionandBiasinASTMTestMethodsE691PracticeforConductinganInterlaboratoryStudytoDeterminethePrecisionofaTestMethod3.SummaryofTestMethod3.1Thed...
