ASTM_D_8442_-_22.pdf

Designation:D844222Standard Test Method forDetermination of Cannabinoids in Cannabis Raw Materialsand Resin Cannabis Products by Gas Chromatography andFlame Ionization Detection1This standard is issued under the fixed designation D8442;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method covers the analysis of cannabinoids incannabis products by gas chromatography(GC)and flameionization detection(FID).1.2 This test method is applicable to cannabis raw materialsand resin cannabis products as defined in Guide D8245,including those from hemp.Such material includes:biomass;plant material;flowers;resins;extracts;distillates;recoveredsolvents;and other intermediate processing material.Theapplicable concentration range of analysis will vary to someextent depending on the nature of the sample,for instancemeasurement of delta-9-tetrahydrocannabinol(9-THC)forregulatory purposes in hemp would require calibration to lowerconcentration levels compared to measurement of CBD in itsisolate;however,in most cases,the test method is applicable tothe determination of major and minor cannabinoids aboveabout 0.1 mass%in concentration.Dilution of sample solutionsis used to adjust concentrations to fall within appropriatecalibration curves.Particular emphasis is placed on the deter-mination of 9-THC for regulatory compliance purposes andcontrol.This test method can measure any cannabinoid that iseluted and detected from a GC column with sufficient resolu-tion from any interfering compounds.Typical cannabinoids ofinterest that can be determined by this test method are shownin Table 1.Use of an HPLC technique is recommended ifindividual measurement of acids,such as THCA,is required.1.3 The test method does not purport to identify all indi-vidual cannabinoids;however,individual users can adapt thistest method for specific custom analyses to meet their needs.1.4 UnitsValues stated in SI units are to be regarded as thestandard.No other units of measurement are included in thisstandard.1.5 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.1.6 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:2D4307 Practice for Preparation of Liquid Blends for Use asAnalytical StandardsD4626 Practice for Calculation of Gas ChromatographicResponse FactorsD8245 Guide for Disposal of Resin-Containing CannabisRaw Materials and Downstream ProductsD8270 Terminology Relating to CannabisD8282 Practice for Laboratory Test Method Validation andMethod DevelopmentD8334/D8334M Practice for Sampling of Cannabis/HempPost-Harvest Batches for Laboratory AnalysesD8375 Test Method for Determination of Cannabinoid Con-centration in Dried Cannabis and Hemp Raw Materialsusing Liquid Chromatography Tandem Mass Spectrom-etry(LC-MS/MS)E355 Practice for Gas Chromatography Terms and Relation-shipsE594 Practice for Testing Flame Ionization Detectors Usedin Gas or Supercritical Fluid ChromatographyE1510 Practice for Installing Fused Silica Open TubularCapillary Columns in Gas Chromatographs3.Terminology3.1 Definitions:3.1.1 Refer to Terminology D8270 for guidance on termi-nology relating to cannabis.1This test method is under the jurisdiction of ASTM Committee D37 onCannabis and is the direct responsibility of Subcommittee D37.03 on Laboratory.Current edition approved July 15,2022.Published October 2022.DOI:10.1520/D8442-22.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.13.1.2 decarboxylation,nremoval of a carboxylate groupfrom a cannabinolic acid usually through application of heat orby exposure to ultraviolet light.3.1.3 distillate,nintermediate or final product resultingfrom separ