Designation:D7558−09(Reapproved2014)StandardTestMethodforColorimetric/SpectrophotometricProceduretoQuantifyExtractableChemicalDialkyldithiocarbamate,Thiuram,andMercaptobenzothiazoleAcceleratorsinNaturalRubberLatexandNitrileGloves1ThisstandardisissuedunderthefixeddesignationD7558;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethodisdesignedtoquantifytheamountoftotalextractableacceleratorsinnaturalrubberlatex(NRL)andnitrilegloves.Threecommonclassesofrubberaccelerators,themercaptobenzothiazole(MBT),thiuram,andthiocarbamatetypecompoundscanbedetectedandquantifiedbythismethod.Ifthespecificrubberaccelerator(s)presentintheglovematerialisnotknown,quantificationisbasedonzincdibutyl-dithiocarbamate(ZDBC)equivalents.Thismethodwillnotdetectallpotentialrubberaccelerators,includingmercaptoben-zothiazoledisulfide,dimorpholine,thioureasanddiphenyldiamine.1.2Forthepurposeofthistestmethod,therangeofchemicalacceleratormeasurementisbasedonthelimitofdetection(LOD)establishedintheperforminglaboratory.1.3Thistestmethodshouldbeperformedbyexperiencedanalystsorunderthesupervisionofthoseexperiencedintheuseofspectroscopyandworkingwithorganicsolvents.1.4Thistestmethodhasnotbeenvalidatedformeasure-mentoflongchaindithiocarbamatesoracceleratorsfromotherrubberproducts,suchaslubricatedcondoms(1).2Althoughthisassayhasbeenreportedintheliteraturefortheevaluationofacceleratorlevelsincondoms,furthervalidationforaccel-eratormeasurementfromotherrubberproductsisrequiredbythetestinglaboratorypriortouse.1.5ThistestmethodisnotdesignedtoevaluatethepotentialofrubbermaterialstoinduceorelicitTypeIVskinsensitiza-tionreactions(forTypeIVskinsensitizationreactionsseeTestMethodD6355).Totalextractableacceleratorcontentdoesnotreflectbioavailablityofindividualacceleratorsthatarede-tectedandmea...
