ASTM_D_7658_-_17_2021.pdf

Designation:D765817(Reapproved 2021)Standard Test Method forDirect Microscopy of Fungal Structures from Tape1This standard is issued under the fixed designation D7658;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method uses optical microscopy for thedetection,semi-quantification,and identification of fungalstructures in tape lift preparations.1.2 This test method describes the preparation techniquesfor tape-lift matrices,the procedure for confirming the pres-ence of fungal structures,and the reporting of observed fungalstructures1.3 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.4 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:2D1193 Specification for Reagent WaterD1356 Terminology Relating to Sampling and Analysis ofAtmospheres3.Terminology3.1 DefinitionsFor definitions of other terms used in thistest method,refer to Terminology D1356.3.2 Definitions of Terms Specific to This Standard:3.2.1 fungal structure(sing.),na collective term for afragment-or groups of fragments from fungi,including but notlimited to conidia,conidiophores,hyphae,and spores.3.2.2 magnification/resolutioncombination1,n100400 total magnification and a point to point resolutionof 0.7 m or better.3.2.3 magnification/resolution combination 2,n 400 orgreater total magnification and a point to point resolution of 0.5m or better.3.2.4 mounting medium,na liquid,for example,lacticacid or prepared stain,used to immerse the sample particulatematter and to attach a cover slip to the sample.3.2.5 tape lift sample,nmaterial lifted from a surfaceusing clear,transparent,single sided,adhesive collectionmedium,typically tape or commercially available preparedslides.4.Summary of Test Method4.1 A tape lift sample is prepared.4.2 The prepared sample is examined on an optical micro-scope for the presence,type and semi-quantification of fungalstructures and reported.5.Significance and Use5.1 The significance of this test method is to standardize theanalysis of the detection of removable fungal structures liftedfrom a surface with tape to improve consistency betweenlaboratories and analysts.5.2 This test method is intended to ensure consistent data tothe end user.5.3 Fungal structures are identified and semi-quantifiedregardless of whether they would or would not grow in culture.5.4 It must be emphasized that the detector in this testmethod is the analyst,and therefore results are subjective,depending on the experience,training,qualification,opticalacuity,and mental fatigue of the analyst.5.5 This test method can be used to assess the presence andcharacteristics of fungal material on a surface.6.Interferences6.1 Look-alike Non-fungal ParticlesCertain types of par-ticles of non-fungal origin may resemble fungal structures.1This test method is under the jurisdiction of ASTM Committee D22 on AirQuality and is the direct responsibility of Subcommittee D22.08 on Assessment,Sampling,and Analysis of Microorganisms.Current edition approved Sept.1,2021.Published September 2021.Originallyapproved in 2017.Last previous edition approved in 2017 as D7658 17.DOI:10.1520/D7658-17R21.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.1These particles and artifacts may include air or plant resin,bubbles,starch,talc or cosmetic particles,or combustionproducts.Non-fungal reference slides(mounted similarly totape-lift samples)should be examined by laboratory analysts toknow how to differentiate such particles.Examination ofsuspect particles using optical