ASTM_D_7789_-_12.pdf

Designation:D778912Standard Practice forCollection of Fungal Material from Surfaces by Swab1This standard is issued under the fixed designation D7789;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 The purpose of this standard practice is to describe theprocedures for collection of surface samples using sterileswabs.1.2 The purpose of this standard practice is to support thefield investigator in differentiating fungal materials from non-fungal material such as scuffs,soot deposits,stains,pigments,dust,efflorescence,adhesives,dust,and water stains.1.3 This practice does not address building occupantexposures,or occupant health risks.1.4 The samples collected by this practice are appropriatefor culture,direct microscopy,and/or biochemical analysis.1.5 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D1356 Terminology Relating to Sampling and Analysis ofAtmospheresD6044 Guide for Representative Sampling for Managementof Waste and Contaminated Media3.Terminology3.1 Definitions:3.1.1 sample,na portion of a population intended to berepresentative of the whole.a portion of a population.Aportionof material that is taken for testing or record purposes.D60443.1.2 sampling,na process consisting of the withdrawal orisolation of a fractional part of the whole.D13563.2 Definitions of Terms Specific to This Standard:3.2.1 area(surface,sampled),na defined,measured ex-tent of surface sampled.3.2.2 biochemical analysis,nact of assessing chemicalsubstances(that is,chitin,enzyme activity,ergosterol,mycotoxin,DNA)originating from living or dead organisms.3.2.3 chain of custody(COC),na document that providesfor the traceable transfer of field samples to the analyticallaboratory.It may or may not be combined with the Field DataSheet.3.2.4 culture analysis,nact of assessing microorganisms(if present)capable of growing on selected media underartificial and controlled conditions.3.2.5 direct microscopy analysis,nact of assessing micro-organisms(if present)using an optical compound microscope.3.2.6 field data sheet,na record of varying names thatprovides a reference document for information directly relatedto the sample collection event,including pre-and post-calibration data.3.2.7 fungi,neukaryotic,heterotrophic,absorptive organ-isms that develop a rather diffuse,branched,tubular body(thatis,network of hyphae)and reproduce by means of spores.3Theterms mold and mildew are frequently used by laypersonswhen referring to various fungal colonization.For purposes ofthis standard,these terms have no technical meaning.3.2.8 fungal material,nfungal spores,hyphae and repro-ductive structures.4.Significance and Use4.1 This practice provides a procedure for collecting surfacematerial using a sterile swab.4.2 A swab sample collected according to this practice isintended to be used to assess fungal material on surfaces.4.3 A swab sample collected from an area with defineddimensions can be used to quantify fungal material per unitarea or for qualitative analysis.4.4 Aswab sample collected from point(s)of interest can beused for qualitative analysis or to quantify fungal material persample.1This practice is under the jurisdiction ofASTM Committee D22 on Air Qualityand is the direct responsibility of Subcommittee D22.08 on Sampling and Analysisof Mold.Current edition approved Aug.15,2012.Published November 2012.DOI:10.1520/D778912.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3Kendrick,Bryce,The Fifth Kingdom,1992,3rd Edition.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 4.5 A swab sample collected according to this standardpractice can be analyzed by direct microscopy,culture,orbiochemical analysis.5.Materials and Supplies5.1 Swabs5.1.1 Must be sterile and have a sterile transport container.5.1.2 May be used dry or wetted.5.1.2.1 Wetting/transport media(not required for dry swabs)must be appropriate for the intended analysis.5.2 Ruler,template or equivalent for measuring sample area.5.3 Permanent marking pen or labels for sample identifica-tion.5.4 Chain of Custody(COC).5.5 Field Data Sheet,if separate from the COC.5.6 Temperature controlled transportation container,as ap-propriate.6.Procedure6.1 Document sample location/surface.6.2 Document the dimensions of the sampled area,ifapprop