Designation:D8070−21StandardTestMethodforScreeningofFuelsandFuelAssociatedAqueousSpecimensforMicrobialContaminationbyLateralFlowImmunoassay1ThisstandardisissuedunderthefixeddesignationD8070;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope*1.1Thistestmethoddescribesaprocedurethatcanbeusedinthefieldorinalaboratorytodetectantigensindicativeofmicrobialcontaminationinliquidfuels,includingthoseblendedwithsynthesizedhydrocarbonsorbiofuels,withkine-maticviscosities(at40°C)of≤24mm2s–1(forexample,SpecificationsD396,D975,andD1655)andinfuel-associatedwater.1.1.1ThistestmethodhasbeenvalidatedbyanILSforarangeofmiddledistillatefuelsmeetingSpecificationD1655,EN590,SpecificationD975,andISO8217:2012.1.2Thistestmethodsemi-quantitativelyassessesthecon-centrationofspecificantigensgeneratedbyaerobicmicroor-ganismsduringactivegrowthinfuels.1.2.1Aproprietaryformulationofantibodiesandantibodymixturesisusedtodetectthreetypesofmicrobialantigencontamination:antigensgenerallyfoundinaerobicbacteria,antigensgenerallypresentincommonfungi(yeastandmolds),andanantigenthatischaracteristicofHormoconisresinae(thefungusmostcommonlyassociatedwithfuelbiodeterioration).1.2.2Althoughtheantibodiesandantibodymixturesarecharacteristicofdiversetypesofbacteriaandfungi,itisunlikelythattheyareuniversal.Recognizingthatforeverymicrobethathasbeenisolatedandcharacterized,itislikelythatthereareabillionthathavenot.Consequently,asisthecasewithallmicrobiologicaltestmethods,thistestmethoddoesnotpurporttodetect100%ofthemicrobespresentinafuelorfuel-associatedwatersample.1.3Foreachofthethreesetsofantigendetected(H.resinae,commonfungi,andaerobicbacteria),thetestdetectswhethertheantigenconcentrationpresentiswithinsetrangesrepresentingnegligible,moderate,orheavymicrobialcontami-nation.1.3.1Forfuelspecimens,thea...
