ASTM_D_8070_-_21.pdf

Designation:D807021Standard Test Method forScreening of Fuels and Fuel Associated AqueousSpecimens for Microbial Contamination by Lateral FlowImmunoassay1This standard is issued under the fixed designation D8070;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope*1.1 This test method describes a procedure that can be usedin the field or in a laboratory to detect antigens indicative ofmicrobial contamination in liquid fuels,including thoseblended with synthesized hydrocarbons or biofuels,with kine-matic viscosities(at 40 C)of 24 mm2s1(for example,Specifications D396,D975,and D1655)and in fuel-associatedwater.1.1.1 This test method has been validated by an ILS for arange of middle distillate fuels meeting Specification D1655,EN590,Specification D975,and ISO 8217:2012.1.2 This test method semi-quantitatively assesses the con-centration of specific antigens generated by aerobic microor-ganisms during active growth in fuels.1.2.1 A proprietary formulation of antibodies and antibodymixtures is used to detect three types of microbial antigencontamination:antigens generally found in aerobic bacteria,antigens generally present in common fungi(yeast and molds),and an antigen that is characteristic of Hormoconis resinae(thefungus most commonly associated with fuel biodeterioration).1.2.2 Although the antibodies and antibody mixtures arecharacteristic of diverse types of bacteria and fungi,it isunlikely that they are universal.Recognizing that for everymicrobe that has been isolated and characterized,it is likelythat there are a billion that have not.Consequently,as is thecase with all microbiological test methods,this test methoddoes not purport to detect 100%of the microbes present in afuel or fuel-associated water sample.1.3 For each of the three sets of antigen detected(H.resinae,common fungi,and aerobic bacteria),the test detectswhether the antigen concentration present is within set rangesrepresenting negligible,moderate,or heavy microbial contami-nation.1.3.1 For fuel specimens,the antigen concentration rangesdetected are 750 g L(heavy).1.3.2 For specimens of water associated with fuel,theantigen concentration ranges detected are 166 g mL(heavy).1.4 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.5 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety,health,and environmental practices and deter-mine the applicability of regulatory limitations prior to use.For a specific hazard statement,see Section 8.1.6 This international standard was developed in accor-dance with internationally recognized principles on standard-ization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recom-mendations issued by the World Trade Organization TechnicalBarriers to Trade(TBT)Committee.2.Referenced Documents2.1 ASTM Standards:2D396 Specification for Fuel OilsD975 Specification for Diesel FuelD1129 Terminology Relating to WaterD1655 Specification for Aviation Turbine FuelsD4175 Terminology Relating to Petroleum Products,LiquidFuels,and LubricantsD4176 Test Method for Free Water and Particulate Contami-nation in Distillate Fuels(Visual Inspection Procedures)D6469 Guide for Microbial Contamination in Fuels and FuelSystems1This test method is under the jurisdiction of ASTM Committee D02 onPetroleum Products,Liquid Fuels,and Lubricants and is the direct responsibility ofSubcommittee D02.14 on Stability,Cleanliness and Compatibility of Liquid Fuels.Current edition approved April 1,2021.Published April 2021.Originallypublished in 2016.Last previous edition approved in 2020 as D8070 20.DOI:10.1520/D8070-21.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.*A Summary of Changes section appears at the end of this standardCopyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United StatesThis international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for theDevelopment of International Standards,Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade(TBT)Committee.1D6974 Practice for Enumeration of Viable Bacteria andFungi in Liquid FuelsFiltration and Culture ProceduresD7464 Practice for Manual Sampling of Liquid Fuels,As-sociated Materials and Fuel System Components forMicrobiological TestingD76