Designation:E2458−10StandardPracticesforBulkSampleCollectionandSwabSampleCollectionofVisiblePowdersSuspectedofBeingBiothreatAgentsfromNonporousSurfaces1ThisstandardisissuedunderthefixeddesignationE2458;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thesepracticesaddresscollectionofvisiblepowdersthataresuspectedbiothreatagentsfromsolidnonporoussurfacesusingabulkcollectionmethod,usingadryswabandlaminatedcard,followedbyaswabsamplingmethodusingasterilemoistenedswab.BulkpowdersamplesarecollectedandpackagedinamannerthatpermitsthemaximumamountofthesampletobesafelytransportedtoareferencelaboratorywithintheCentersforDiseaseControlandPrevention(CDC)nationalLaboratoryResponseNetwork(LRN)2forconfirmatoryiden-tificationandsafestorage.Ifthesourceofthepowderisaletterorsmallpackage,thatitemisalsopackagedinamannerthatpermitsittobesafelytransportedtoanLRNreferencelaboratory.Asterilemoistenedswabmaybeusedtocollectresidualpowderandmaybeusedtoconducton-sitebiologicalassessmentsforthepurposeoftestingforbiothreatagents.1.2ThesepracticesareperformedincoordinationwiththeFederalBureauofInvestigation(FBI)aspartofariskassessmentincludinghazardassessmentandthreatevaluationasrecommendedandclarifiedinGuideE2770.ThedecisiontoimplementthesepracticesandcollectapublicsafetysamplewillbemadebymembersoftheresponsecommunityofthejurisdictionassumingresponsibilitythroughcoordinationwiththeFBIandthereceivingLRNreferencelaboratory.1.3SampleCollectionMethodAcoversthebulkcollectionandpackagingofsuspiciousvisiblepowdersthataresuspectedbiothreatagentsfromsolidnonporoussurfaces.AllsamplessuspectedtobebiothreatagentsonnonporoussurfacesshouldbecollectedaccordingtoSampleCollectionMethodAandsenttoaLRNreferencelaboratoryforconfirmatorytesting.1.4SampleCollectionMethodBcoversswabsamplingofresidualsu...
