ASTM_E_2274_-_16.pdf

Designation:E227416Standard Test Method forEvaluation of Laundry Sanitizers and Disinfectants1This standard is issued under the fixed designation E2274;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method is designed to evaluate sanitizing/disinfectant laundry detergents/additives for use in traditionaltop-loading automatic clothes washing operations.This testmethod is designed predominantly to provide testing withrepresentative vegetative bacteria but can also be designed toaccommodate the testing of fungi and viruses.NOTE1This test method does not evaluate sanitizing/disinfectantlaundry detergent/additives for use in front-loading or top-loading,lowwater high efficiency automatic clothes washing operations.1.2 This test method is intended to compliment Test MethodE2406 and is to be used in the cases where this test method isdetermined to be the worse case scenario for product usage.1.3 Knowledge of microbiological techniques is requiredfor these procedures.1.4 It is the responsibility of the investigator to determinewhether Good Laboratory Practices(GLP)are required and tofollow them where appropriate(see section 40 CFR,160 or asrevised.)1.5 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.NOTE2In this test method,metric units are used for all applications,except for distance in which case inches are used.1.6 Appropriate modifications to the test method may berequired when the testing organisms are not specified herein.1.7 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D1193 Specification for Reagent WaterE177 Practice for Use of the Terms Precision and Bias inASTM Test MethodsE691 Practice for Conducting an Interlaboratory Study toDetermine the Precision of a Test MethodE1054 Test Methods for Evaluation of Inactivators of Anti-microbial AgentsE2406 Test Method for Evaluation of Laundry Sanitizersand Disinfectants for Use in High Efficiency WashingOperationsE2756 Terminology Relating to Antimicrobial and AntiviralAgents2.2 Other Standards:AATCC Test Method 70 Water Repellency;Tumble JarDynamic Absorption Test3OCSPP 810.2400:Disinfectants and Sanitizers for Use onFabrics and Textiles Efficacy Data Recommendations440 CFR,Part 160,Good Laboratory Practice Standards53.Terminology3.1 For definitions of terms used in this test method refer toTerminology E2756.3.2 Definitions of Terms Specific to This Standard:3.2.1 active antimicrobial ingredienta substance added toa formulation intended specifically for the inhibition or inac-tivation of microorganisms.1This test method is under the jurisdiction of ASTM Committee E35 onPesticides,Antimicrobials,and Alternative Control Agents and is the directresponsibility of Subcommittee E35.15 on Antimicrobial Agents.Current edition approved April 15,2016.Published May 2016.Originallyapproved in 2003.Last previous edition approved in 2009 as E2247 09.DOI:10.1520/E2274-16.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3Available from American Association of Textile Chemists and Colorists(AATCC),P.O.Box 12215,Research Triangle Park,NC 27709,http:/www.aatcc.org.4Available from United States Environmental ProtectionAgency(EPA),WilliamJefferson Clinton Bldg.,1200 Pennsylvania Ave.,NW,Washington,DC 20460,http:/www.epa.gov.5Available from U.S.Government Publishing Office Bookstore 710 NorthCapitol Street N.W.Washington,DC,http:/www.gpo.gov/about/bookstore.htmCopyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 3.2.2 antimicrobial agent(s)an active ingredient designedto suppress the growth or action of microorganisms.3.2.3 carrier count controlprocedure used to determinethe initial number of microorganisms on a fabric carrierfollowing the inoculation and drying procedure.3.2.4 diluentsterile deionized water,sterile distilled wateror sterile synthetic AOAC hard water that may be used toprepare the active test formulation,vehicle control or productcontrol for use in the test procedure.3.2.5 diluted product solutiontest formulation,vehiclecontrol,or product control diluted to use concentration.3.2.6 numberscontrolinassessingsanitizerlevelperformance,procedure used to determine the number ofmicroorganisms remaining on the fabric carriers and in the